STOCK Tg(Pdx1-cre/Esr1*)#Dam/J

Stock number: 024968
Product name: Pdx1-CreER
Research areas: Research Tools

Description

This tamoxifen-inducible Pdx1-Cre/Esr1 strain may be used to create conditional pancreatic cell knockouts when crossed with strains carrying floxed alleles.

Detailed description

Pancreatic cells are derived from Pdx1 (pancreatic and duodenal homeobox 1)-expressing progenitors throughout embryogenesis.

This conditional transgenic strain expresses Cre/Esr1 from the mouse Pdx1 promoter. Cytoplasmic stores of Cre recombinase translocate to the nuclear compartment upon induction with tamoxifen. When crossed with a strain containing a loxP-flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Immunohistochemistry demonstrates that transgene expression closely follows endogenous Pdx1 patterns. Expression is observed in acini, islet and duct cells but not in mesenchymal cells (endothelial and mesenchyme-derived smooth muscle cells) of the pancreas. Robust cre expression can be detected by E10.5 in pancreatic epithelium.

Female mice crossed with Z/AP floxed reporter males (see Stock No. 003919) and then injected with tamoxifen demonstrate that the drug is active for less than 48 hours, allowing one to mark progeny of PDX1+ cells indelibly at any stage of development.

Development

Cre-ER^TM (Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) was introduced to the starting ATG of mouse Pdx1, and followed by an SV40 polyadenylation signal. The transgenic vector was injected into B6CBAF1 embryos. Resultant mice were originally crossed with outbred ICR or CD1 animals, then backcrossed to C57BL/6. Subsequent crosses to both ICR/CD1 and C57BL/6 contribute to the STOCK background.

Allele

Symbol: Tg(Pdx1-cre/Esr1*)#Dam
Name: transgene insertion, Douglas A Melton
MGI accession ID: MGI:5008261
Generation method: Transgenic
Attributes: Recombinase-expressing; Inducible
Marker symbol: Tg(Pdx1-cre/Esr1*)#Dam
Marker name: transgene insertion, Douglas A Melton
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: cre/Esr1,Cre recombinase and estrogen receptor 1 fusion gene,
Site of expression: Tamoxifen induction drives mosaic expression of cre recombinase in endocrine, exocrine, and duct tissue of the pancreas.
Molecular note: The sequence encoding a tamoxifen-inducible form of a cre recombinase/estrogen receptor fusion protein was adjoined to the Ipf1 promoter to drive mosaic expression of cre recombinase in endocrine, exocrine, and duct tissue of the pancreas. The pound symbol (#) is used when no line is specified and/or lines are pooled.