These Chst10 knockout mice are viable, but subfertile as a result of steroid hormone dysregulation. Chst10 encodes a carbohydrate sulfotransferase that adds sulfate to glucuronic acid and may be useful for studying the regulation of steroid hormones and neuronal cell interactions.
Minoru Fukuda, Sanford-Burnham Medical Research Institute
Chst10 encodes a carbohydrate sulfotransferase that adds sulfate to glucuronic acid. CHST10 is involved in the biosynthesis of HNK1 (human natural killer 1) a carbohydrate found in neurons and NK cells. CHST10 also sulfates glucuronidated estrogen, testosterone and other steroid hormones. Mice homozygous for this knockout allele are viable, but subfertile. Females exhibit a thickened uterine endometrium, high serum estrogen levels and an enlarged uterus at the pro-estrus stage. Homozygous x wild type crosses using mutant males or females are either non-productive or result in small litter sizes. This strain may be useful for studying the regulation of steroid hormones and neuronal cell interactions.
A targeting vector containing the neomycin resistance was used to disrupt exon 5, which contains the enzyme catalytic domain. The construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and offspring were backcrossed to C57BL/6 for at least 7 generations by the donating lab (see SNP note below). The donating investigator indicates that the ES cell line used was probably R1 (129X1/SvJ x 129S1/Sv)F1, however, there is a possibility that it was D3 (129S2/SvPas).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Ten markers throughout the genome were found to be segregating with 129, suggesting an incomplete backcross.
|Allele Name||targeted mutation 1, Minoru Fukuda|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Chst10, carbohydrate sulfotransferase 10|
|Strain of Origin||129|
|Molecular Note||A neo cassette replaced the coding sequence in exon 5. Immunohistochemistry confirmed the absence of protein expression in the hippocampus.|
While maintaining a live colony, these mice are bred as heteroygotes. Mice homozygous for the mutation are subfertile. The donating investigator indicates that extended backcrossing of heterozygotes to C57BL/6 mice results in non-productive matings.
When using the Chst10- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37129 in your Materials and Methods section.