C57BL/6J-Tg(tetO-EGFP/Rpl10a)5aReij/J

Stock number: 024898
Research areas: Neurobiology Research, Research Tools

Description

These tetO-EGFP-L10a mice carry a EGFP/Rpl10a fusion gene, regulated by a tetracycline operator (tetO). When crossed with a mutant strain expressing tetracycline-controlled transactivator protein (tTA), this strain allows identification and collection of specific changes in local mRNA populations by ribosome immunoprecipitation.

Detailed description

Expression of the epitope-tagged EGFP/Rpl10a fusion gene (EGFP fused to the N-terminus of the large subunit ribosomal protein L10a) is regulated by a tetracycline operator (tetO) in this transgenic strain. When mated to a strain expressing tetracycline-controlled transactivator protein (tTA), expression of EGFP/Rpl10a fusion protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. Expression of EGFP/Rpl10a within a cell type allows identification and collection of cell type-specific changes in mRNA populations by ribosome immunoprecipitation, where recovery of intact cells for analysis is not possible. Mice that are hemizygous for the transgene are viable and fertile.

When crossed with a Camk2a-tTA strain that expresses tTA in forebrain neurons (see Stock No. 007004, for example), ribosome-bound mRNA from CA1 pyramidal neuron dendrites can be isolated and collected by immunoprecipitating EGFP-tagged ribosomes.

Development

A transgenic construct containing sequence encoding EGFP/Rpl10a fusion protein (EGFP fused to the N-terminus of the large subunit ribosomal protein L10a) under the control of the tetO, tetracycline-responsive regulatory element, was injected into fertilized C57BL/6J mouse eggs. Founder line 5a was subsequently established. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(tetO-EGFP/Rpl10a)5aReij
Name: transgene insertion 5a, Leon Reijmers
MGI accession ID: MGI:5582628
Generation method: Transgenic
Attributes: Reporter; Inducible; Inserted expressed sequence
Marker symbol: Tg(tetO-EGFP/Rpl10a)5aReij
Marker name: transgene insertion 5a, Leon Reijmers
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: GFP,Green Fluorescent Protein,; Rpl10a,ribosomal protein L10A,mouse, laboratory
Site of expression: Expression of EGFP/Rpl10a fusion gene is regulated with tetracycline when these mice are mated to a mutant expressing tTA.
Molecular note: A transgenic construct containing sequence encoding EGFP/Rpl10a fusion protein (EGFP fused to the N-terminus of the large subunit ribosomal protein L10a) under the control of the tetO, tetracycline-responsive regulatory element.