The PS19 mouse model harbors the T34 isoform of microtubule-associated protein tau with one N-terminal insert and four microtubule binding repeats (1N4R) encoding the human P301S mutation, all driven by the mouse prion protein promoter. These mice are useful in studying neurofibrillary tangles, neurodegenerative tauopathy and Alzheimer's disease.
Virginia M Lee, University of Pennsylvania
The P301S transgene was designed with the P301S mutant human microtubule-associated protein tau (MAPT*P301S) under the direction of the mouse prion protein promoter (Prnp). This transgene was injected into fertilized B6C3F1 mouse eggs. Transgenic founder animals were bred to B6C3F1/J or B6C3F1/Crl mice to establish founder line 19 (PS19). The resulting PS19 colony was maintained in a hemizygous fashon on the B6C3F1 background, and then backcrossed to C57BL/6J for 10 generations. The mice were then maintained by crossing transgenic males to to C57Bl/6NCrl females for 7 generations. During backcrossing, the Y chromosome was not fixed to the C57Bl/6NCrl genetic background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.
|Expressed Gene||MAPT, microtubule associated protein tau, human|
|Site of Expression|
|Allele Name||transgene insertion PS19, Virginia M Y Lee|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||P301S tau (line PS19); PS19 Tg|
|Gene Symbol and Name||Tg(Prnp-MAPT*P301S)PS19Vle, transgene insertion PS19, Virginia M Y Lee|
|Promoter||Prnp, prion protein, mouse, laboratory|
|Expressed Gene||MAPT, microtubule associated protein tau, human|
|Strain of Origin||(C57BL/6 x C3H)F1|
|Molecular Note||The transgene construct contains the P301S mutant human microtubule-associated protein tau, MAPT, under the direction of the mouse prion protein, Prnp, promoter. The expression of the mutant human MAPT is 5-fold higher than the expression of the endogenous mouse Mapt protein. Transgene insertion occurred on Chr 3, causing a 249 Kb deletion.|
|Mutations Made By|| |
Virginia Lee, University of Pennsylvania
When maintaining a live colony, hemizygous mice may be bred together, to wildtype siblings, or to C57BL/6NJ inbred mice (Stock No. 005304).
It should be noted that homozygous females fail to mate successfully.
When using the B6N.Cg-Tg(Prnp-MAPT*P301S)PS19Vle/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024841 in your Materials and Methods section.
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