Tg11.5kb-GFP transgenic mice have 11.5kb of the inositol polyphosphate-5-phosphatase D, or Ship promoter driving expression of EGFP in presumptive stem cells in various tissues during development.
Jonathan A. Cooper, Fred Hutchinson Cancer Research Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Tg11.5kb-GFP transgenic mice have 11.5kb of the inositol polyphosphate-5-phosphatase D (Inpp5d) gene, containing the internal promoter driving expression of an enhanced green fluorescent protein (EGFP). SHIP1 is a negative regulator of myeloid and lymphoid cell proliferation, survival, and chemotaxis. The internal promoter normally expresses a form of SHIP1, known as s-SHIP, that lacks the N-terminal SH2 domain. Homozygotes are viable and fertile. Under control of this s-Ship promoter, EGFP labels presumptive stem cells in various tissues including the blastocyst, primordial germ cells, thymus, arterioles, osteoblasts, mammary bud cells, cap cells in the puberty mammary gland, basal alveolar bud cells in mammary glands during pregnancy, and skin epidermis.
The Tg11.5kb-GFP transgene was designed with an enhanced green fluorescent protein (EGFP) driven by 11.5kb of the inositol polyphosphate-5-phosphatase D (Inpp5d) promoter, followed by a polyadenylation site. The transgene was microinjected into fertilized (C57BL/6 x CBA/J)F1 oocytes and mice from founder line D were bred to C57BL/6J mice for at least 11 generations. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP is expressed in presumptive stem cells in various tissues including the blastocyst, primordial germ cells, thymus, arterioles, osteoblasts, mammary bud cells, cap cells in the puberty mammary gland, basal alveolar bud cells in mammary glands during pregnancy, and skin epidermis. |
Allele Name | transgene insertion D, Larry R Rohrschneider |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Tg 11.5-kb GFP |
Gene Symbol and Name | Tg(Inpp5d-EGFP)DLrr, transgene insertion D, Larry R Rohrschneider |
Gene Synonym(s) | |
Promoter | Inpp5d, inositol polyphosphate-5-phosphatase D, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is expressed in presumptive stem cells in various tissues including the blastocyst, primordial germ cells, thymus, arterioles, osteoblasts, mammary bud cells, cap cells in the puberty mammary gland, basal alveolar bud cells in mammary glands during pregnancy, and skin epidermis. |
Strain of Origin | (C57BL/6 x CBA/J)F1 |
Chromosome | UN |
Molecular Note | The Tg 11.5kb-GFP transgene was designed with an enhanced green fluorescent protein (EGFP) driven by 11.5kb of the inositol polyphosphate-5-phosphatase D (Inpp5d) promoter, followed by a polyadenylation site. Lines A,B C and D were established, however, Line B lacks expression of the transgene. Results are primarily from Line D, although all three lines have similar in vivo expression patterns. Line D has 11 copies. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Tg 11.5-kb GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #024808 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizyous for Tg(Inpp5d-EGFP)DLrr |
Frozen Mouse Embryo | B6.Cg-Tg(Inpp5d-EGFP)DLrr/CprJ | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Inpp5d-EGFP)DLrr/CprJ | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Inpp5d-EGFP)DLrr/CprJ | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Inpp5d-EGFP)DLrr/CprJ | $3373.50 |
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