B6.Cg-Tg(Inpp5d-EGFP)DLrr/CprJ

Stock number: 024808
Product name: Tg 11.5-kb GFP
Research areas: Developmental Biology Research, Research Tools

Description

Tg11.5kb-GFP transgenic mice have 11.5kb of the inositol polyphosphate-5-phosphatase D, or Ship promoter driving expression of EGFP in presumptive stem cells in various tissues during development.

Detailed description

Tg11.5kb-GFP transgenic mice have 11.5kb of the inositol polyphosphate-5-phosphatase D (Inpp5d) gene, containing the internal promoter driving expression of an enhanced green fluorescent protein (EGFP). SHIP1 is a negative regulator of myeloid and lymphoid cell proliferation, survival, and chemotaxis. The internal promoter normally expresses a form of SHIP1, known as s-SHIP, that lacks the N-terminal SH2 domain. Homozygotes are viable and fertile. Under control of this s-Ship promoter, EGFP labels presumptive stem cells in various tissues including the blastocyst, primordial germ cells, thymus, arterioles, osteoblasts, mammary bud cells, cap cells in the puberty mammary gland, basal alveolar bud cells in mammary glands during pregnancy, and skin epidermis.

Development

The Tg11.5kb-GFP transgene was designed with an enhanced green fluorescent protein (EGFP) driven by 11.5kb of the inositol polyphosphate-5-phosphatase D (Inpp5d) promoter, followed by a polyadenylation site. The transgene was microinjected into fertilized (C57BL/6 x CBA/J)F1 oocytes and mice from founder line D were bred to C57BL/6J mice for at least 11 generations. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.

Allele

Symbol: Tg(Inpp5d-EGFP)DLrr
Name: transgene insertion D, Larry R Rohrschneider
MGI accession ID: MGI:5551779
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Inpp5d-EGFP)DLrr
Marker name: transgene insertion D, Larry R Rohrschneider
Chromosome: UN
Strain of origin: (C57BL/6 x CBA/J)F1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP is expressed in presumptive stem cells in various tissues including the blastocyst, primordial germ cells, thymus, arterioles, osteoblasts, mammary bud cells, cap cells in the puberty mammary gland, basal alveolar bud cells in mammary glands during pregnancy, and skin epidermis.
Molecular note: The Tg 11.5kb-GFP transgene was designed with an enhanced green fluorescent protein (EGFP) driven by 11.5kb of the inositol polyphosphate-5-phosphatase D (Inpp5d) promoter, followed by a polyadenylation site. Lines A,B C and D were established, however, Line B lacks expression of the transgene. Results are primarily from Line D, although all three lines have similar in vivo expression patterns. Line D has 11 copies.