Overview

Also Known As:Aqp11^flx

This loxP-flanked aquaporin 11 (Aqp11) strain may be useful for studying the role of aquaporin 11 in liver and other organs.

Donating Investigator

Aleksandra Rojek, Aarhus University

Genetic overview

Genetic Background	Generation

Aqp11^tm1.1Nlsn

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Aqp11	aquaporin 11

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Research Applications

Research Tools

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Details

Detailed Description

Aquaporin 11 (Aqp11 ) encodes a membrane channel of undetermined function that colocalizes with markers of the rough endoplasmic reticulum (RER). These mice possess loxP sites flanking exons 2 and 3. Mice that are homozygous for this allele are viable and fertile. When crossed to mice carrying Tg(Alb-cre)21Mgn, Cre recombinase expression in the liver has little effect beyond a minor defect in lipid handling in unchallenged mice. However, challenge by fasting/re-feeding or administration of high dose free amino acids results in RER-derived vacuoles in periportal hepatocytes.
Complete deficiency of Aqp11 causes death by 2-8 weeks of age due to kidney malfunction.
This conditional knockout may be useful for studying the role of aquaporin 11 in liver and other organs.

Development

The targeting vector contains a loxP site upstream of exon 2 and a loxP site downstream of exon 3 followed by a frt-flanked PGKneo cassette. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Transient infection with a FLPe plasmid excised the neomycin cassette. The resulting chimeric animals were crossed to C57BL/6 mice. The strain is maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J (Stock No. 000664) mice for at least 1 generation to establish the colony.

Control Suggestions

Approximate Controls

101045 B6129SF2/J

Additional Information

Considerations for Choosing Controls

Selected References

Rojek A; Fuchtbauer EM; Fuchtbauer A; Jelen S; Malmendal A; Fenton RA; Nielsen S. 2013. Liver-specific Aquaporin 11 knockout mice show rapid vacuolization of the rough endoplasmic reticulum in periportal hepatocytes after amino acid feeding. Am J Physiol Gastrointest Liver Physiol 304(5):G501-15PubMed: 23275615 MGI: J:194939

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Genetics

Aqp11^tm1.1Nlsn

Allele Symbol: Aqp11^tm1.1Nlsn

Allele Name	targeted mutation 1.1, Soren Nielsenk
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	Aqp11^flx; Aqp11^tm1.1Aroj
Gene Symbol and Name	Aqp11, aquaporin 11
Gene Synonym(s)
Strain of Origin	129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Chromosome	7
Molecular Note	A loxP site was inserted upstream of exon 2. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the neo cassette and left exons 2 and 3 floxed.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Metabolism Research
- Cre-lox System
  - loxP-flanked Sequences

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Aqp11^tm1.1Nlsn/Aqp11^tm1.1Nlsn Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺
involves: 129S1/Sv * C57BL/6 * DBA

cellular phenotype

abnormal endoplasmic reticulum morphology
- dilated in the hepatocytes of mice that were fasted and re-fed

liver/biliary system phenotype

abnormal hepatocyte morphology
- re-fed mice exhibit rapid and variable development of giant vacuoles (dilated rough endoplasmic reticulum) in periportal hepatocytes unlike in control mice
- following a 24 h fast, hepatocytes surrounding the portal veins are more eosinophilic with vacuolization than in perivenous hepatocytes unlike in control mice
- fasted mice treated with ammonium acetate or pyruvate exhibit some degree of vacuolization unlike control mice
- administration of amino acids to fasted mice exhibit varying degrees of hepatocyte vacuolization depending on the amino acid (glutamine, glutamate, glycine>alanine>arginine>lysine) unlike in control mice
- Normal - however, oral administration of glucose, urea or triglyceride to fasted mice does not induce vacuolization
- orally administration of casein to fasted mice exhibit vacuolization of periportal hepatocytes unlike in control mice

mammalian phenotype

homeostasis/metabolism phenotype
- Normal - mice exhibit normal glucose homeostasis under fed, fasted and re-fed conditions

homeostasis/metabolism phenotype

increased circulating aspartate transaminase level
- in fasted mice

decreased circulating glycerol level
- in fasted mice

decreased blood urea nitrogen level
- in unchallenged mice

increased circulating HDL cholesterol level
- in unchallenged mice

decreased circulating ketone body level
- in unchallenged and fasted mice

decreased circulating triglyceride level
- in fasted mice

decreased circulating free fatty acids level
- in fasted mice

References

Rojek A; Fuchtbauer EM; Fuchtbauer A; Jelen S; Malmendal A; Fenton RA; Nielsen S. 2013. Liver-specific Aquaporin 11 knockout mice show rapid vacuolization of the rough endoplasmic reticulum in periportal hepatocytes after amino acid feeding. Am J Physiol Gastrointest Liver Physiol 304(5):G501-15PubMed: 23275615 MGI: J:194939

Additional - Aqp11^tm1.1Nlsn related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Aqp11
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the Aqp11^flx mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37110 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Aqp11flx

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Aqp11tm1.1Nlsn

Allele Symbol: Aqp11tm1.1Nlsn

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Aqp11tm1.1Nlsn/Aqp11tm1.1Nlsn Speer6-ps1Tg(Alb-cre)21Mgn/Speer6-ps1+involves: 129S1/Sv * C57BL/6 * DBA

cellular phenotype

liver/biliary system phenotype

mammalian phenotype

homeostasis/metabolism phenotype

References

Additional - Aqp11tm1.1Nlsn related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Also Known As:Aqp11^flx

Aqp11^tm1.1Nlsn

Allele Symbol: Aqp11^tm1.1Nlsn

Genotype: Aqp11^tm1.1Nlsn/Aqp11^tm1.1Nlsn Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺
involves: 129S1/Sv * C57BL/6 * DBA

Additional - Aqp11^tm1.1Nlsn related