Foxo4L/L floxed mice may be useful for studying the regulatory role of the FOXO4 transcription factor on insulin signaling and tumor suppression.
Dr. Ronald DePinho, MD Anderson Cancer Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Foxo4 | forkhead box O4 |
These Foxo4L/L mutant mice possess loxP sites flanking exon 1 of the forkhead box O4 (Foxo4) gene. FOXO4 is a transcription factor involved in the regulation of insulin signaling, and is involved in tumor suppression by promoting cell cycle arrest and apoptosis. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 1 deleted in the cre-expressing tissue, resulting in inactivation of Foxo4 gene expression.
A targeting vector was designed to insert a loxP site upstream of exon 1, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 1 of the forkhead box O4 (Foxo4) gene. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to Tg(ACTFLPe)9205Dym transgenic mice to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene. Mice were bred to FVB mice for at least 3 generations before being bred with mice carrying Foxo1tm1Rdp and Foxo3tm1Rdp. Upon arrival, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony. The three alleles in this strain were separated and maintained as separate strains (for Foxo1tm1Rdp see Stock No. 024756 and for Foxo3tm1Rdp see Stock No. 024668). Single mutants were bred to FVB/NJ for at least one more generation.
Allele Name | targeted mutation 1, Ronald DePinho |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Foxo4F; FoxO4L |
Gene Symbol and Name | Foxo4, forkhead box O4 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | X |
Molecular Note | Exon 1 was flanked with loxP sites. This is the second major coding exon and encodes the C-terminal half of the full-length protein. |
Foxo4 is X-linked. When maintaining a live colony, homozygous females may be bred to heterozygous males.
When using the FoxO4L mouse strain in a publication, please cite the originating article(s) and include JAX stock #024757 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X linked - females are Heterozygous and males are wildtype for Foxo4<tm1Rdp>, 1 pair minimum |
Frozen Mouse Embryo | FVB.129S6(Cg)-Foxo4<tm1Rdp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.129S6(Cg)-Foxo4<tm1Rdp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB.129S6(Cg)-Foxo4<tm1Rdp>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB.129S6(Cg)-Foxo4<tm1Rdp>/J Frozen Embryo | $3373.50 |
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