FVB.129S6(Cg)-Foxo4^tm1Rdp/J

Stock number: 024757
Product name: FoxO4^L
Strain synonyms: Foxo4^f
Research areas: Apoptosis Research, Cell Biology Research, Diabetes and Obesity Research, Research Tools

Description

Foxo4^L/L floxed mice may be useful for studying the regulatory role of the FOXO4 transcription factor on insulin signaling and tumor suppression.

Detailed description

These Foxo4^L/L mutant mice possess loxP sites flanking exon 1 of the forkhead box O4 (Foxo4) gene. FOXO4 is a transcription factor involved in the regulation of insulin signaling, and is involved in tumor suppression by promoting cell cycle arrest and apoptosis. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 1 deleted in the cre-expressing tissue, resulting in inactivation of Foxo4 gene expression.

Development

A targeting vector was designed to insert a loxP site upstream of exon 1, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 1 of the forkhead box O4 (Foxo4) gene. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to Tg(ACTFLPe)9205Dym transgenic mice to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene. Mice were bred to FVB mice for at least 3 generations before being bred with mice carrying Foxo1^tm1Rdp and Foxo3^tm1Rdp. Upon arrival, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony. The three alleles in this strain were separated and maintained as separate strains (for Foxo1^tm1Rdp see Stock No. 024756 and for Foxo3^tm1Rdp see Stock No. 024668). Single mutants were bred to FVB/NJ for at least one more generation.

Allele

Symbol: Foxo4^tm1Rdp
Name: targeted mutation 1, Ronald DePinho
MGI accession ID: MGI:3698869
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Foxo4
Marker name: forkhead box O4
Chromosome: X
Strain of origin: 129S6/SvEvTac
Molecular note: Exon 1 was flanked with loxP sites. This is the second major coding exon and encodes the C-terminal half of the full-length protein.