GRTH 6.4Kb/del-Tg transgenic mice have Ddx25 promoter/enhancer sequences driving expression of EGFP in germ cells. These mice may be useful when studying androgen regulation of GRTH.
Maria Dufau, NIH
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
GRTH 6.4Kb/del-Tg transgenic mice have mutated DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences driving expression of an enhanced green fluorescent protein, EGFP. DDX25, also known as gonadotropin-regulated testicular RNA helicase (GRTH), is a testis-specific posttranscriptional regulator of genes that are essential for spermatid elongation and completion of spermiogenesis. These mice contain a 5’ deletion - 3.6 kb/-205 bp upstream of the Ddx25 promoter. Hemizygotes are viable and fertile. GRTH 6.4Kb/del-Tg mice express EGFP only in germ cells. When treated with androgen receptor antagonist, flutamide, to obliterate the action of endogenous circulating testosterone, EGFP expression is significantly decreased in germ cells. Similar to this strain, GRTH 6.4Kb-Tg transgenic mice (Stock No. 024752) have Ddx25 5’ sequences intact, and express EGFP in somatic Leydig cells and germ cells. These mice are more sensitive to flutamide treatment, with no EGFP expression after treatment.
The GRTH 6.4Kb/del-Tg transgene was designed in the laboratory of Dr. Maria L Dufau (Molecular Endocrinology NICHD, NIH) with an enhanced green fluorescent protein, EGFP, driven by mutated DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences. This allele contains a 5’ deletion - 3.6 kb/-205 bp upstream of the Ddx25 promoter. The transgene was microinjected into fertilized C57BL/6N oocytes and founder mice were bred to C57BL/6N inbred mice for at least 15 generations to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP is expressed only in germ cells. |
Allele Name | transgene insertion 1, Maria L Dufau |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | 6.4kb/del-Tg |
Gene Symbol and Name | Tg(Ddx25*-EGFP)1Mld, transgene insertion 1, Maria L Dufau |
Gene Synonym(s) | |
Promoter | Ddx25, DEAD (Asp-Glu-Ala-Asp) box polypeptide 25, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is expressed only in germ cells. |
Strain of Origin | C57BL/6N |
Chromosome | UN |
Molecular Note | The transgene contains an enhanced green fluorescent protein, EGFP, driven by DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences that contains a 5'deletion - 3.6 kb/-205 bp upstream of the promoter. GFP expression is directed to meiotic and haploid germ cells. |
When maintaining a live colony, hemizygous mice may be bred with wildtype (non-carrier) mice from the colony. The donating investigator has not attempted to make this strain homozygous.
When using the C57BL/6N-Tg(Ddx25*-EGFP)1Mld/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024753 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizyous or Non Carrir for Tg(Ddx25*-EGFP)1Mld |
Frozen Mouse Embryo | C57BL/6N-Tg(Ddx25*-EGFP)1Mld/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6N-Tg(Ddx25*-EGFP)1Mld/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6N-Tg(Ddx25*-EGFP)1Mld/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6N-Tg(Ddx25*-EGFP)1Mld/J | $3373.50 |
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