C57BL/6N-Tg(Ddx25*-EGFP)1Mld/J

Stock number: 024753
Research areas: Developmental Biology Research, Reproductive Biology Research, Research Tools

Description

GRTH 6.4Kb/del-Tg transgenic mice have Ddx25 promoter/enhancer sequences driving expression of EGFP in germ cells. These mice may be useful when studying androgen regulation of GRTH.

Detailed description

GRTH 6.4Kb/del-Tg transgenic mice have mutated DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences driving expression of an enhanced green fluorescent protein, EGFP. DDX25, also known as gonadotropin-regulated testicular RNA helicase (GRTH), is a testis-specific posttranscriptional regulator of genes that are essential for spermatid elongation and completion of spermiogenesis. These mice contain a 5’ deletion - 3.6 kb/-205 bp upstream of the Ddx25 promoter. Hemizygotes are viable and fertile. GRTH 6.4Kb/del-Tg mice express EGFP only in germ cells. When treated with androgen receptor antagonist, flutamide, to obliterate the action of endogenous circulating testosterone, EGFP expression is significantly decreased in germ cells. Similar to this strain, GRTH 6.4Kb-Tg transgenic mice (Stock No. 024752) have Ddx25 5’ sequences intact, and express EGFP in somatic Leydig cells and germ cells. These mice are more sensitive to flutamide treatment, with no EGFP expression after treatment.

Development

The GRTH 6.4Kb/del-Tg transgene was designed in the laboratory of Dr. Maria L Dufau (Molecular Endocrinology NICHD, NIH) with an enhanced green fluorescent protein, EGFP, driven by mutated DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences. This allele contains a 5’ deletion - 3.6 kb/-205 bp upstream of the Ddx25 promoter. The transgene was microinjected into fertilized C57BL/6N oocytes and founder mice were bred to C57BL/6N inbred mice for at least 15 generations to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) to establish the colony.

Allele

Symbol: Tg(Ddx25*-EGFP)1Mld
Name: transgene insertion 1, Maria L Dufau
MGI accession ID: MGI:5568393
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Ddx25*-EGFP)1Mld
Marker name: transgene insertion 1, Maria L Dufau
Chromosome: UN
Strain of origin: C57BL/6N
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP is expressed only in germ cells.
Molecular note: The transgene contains an enhanced green fluorescent protein, EGFP, driven by DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) promoter/enhancer sequences that contains a 5'deletion - 3.6 kb/-205 bp upstream of the promoter. GFP expression is directed to meiotic and haploid germ cells.