STOCK Myl1^tm1(cre)Sjb/J

Stock number: 024713
Research areas: Research Tools

Description

These mice express cre recombinase from the skeletal muscle-specific myosin, light polypeptide 1 promoter.

Detailed description

These mice strongly express cre recombinase from the skeletal muscle-specific Myl1 (myosin, light polypeptide 1) promoter. When test crossed with an Erbb4 floxed strain, sequences were deleted selectively in skeletal muscle tissue, including gastrocnemius, tibialis anterior and biceps femoris muscles, and not in brain, liver, heart or stomach.

Development

Cre, carrying a nuclear localization signal (nls), and a loxP-flanked PGK-neo cassette were inserted immediately upstream of the initiation ATG in exon 1 of the Myl1 (myosin, light polypeptide 1) gene. The mutation was created by homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. PGK-neo was removed by crossing resultant mice with deleter CMV-cre animals. This strain was maintained on a mixed genetic background incorporating 129, C57BL/6 and FVB by the donating laboratory.

Allele

Symbol: Myl1^tm1(cre)Sjb
Name: targeted mutation 1, Steven J Burden
MGI accession ID: MGI:1931135
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Myl1
Marker name: myosin, light polypeptide 1
Chromosome: 1
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Mice carrrying this allele express cre recombinase in skeletal muscle tissue, including gastrocnemius, tibialis anterior and biceps femoris muscles.
Molecular note: Insertion of an NLS-cre gene into exon 1, immediately upstream of the initiator ATG. An adjacent lox-P flanked neomycin cassette was removed by Cre mediated recombination in the final allele.