These mice express cre recombinase from the skeletal muscle-specific myosin, light polypeptide 1 promoter.
Jeffery D. Molkentin, Cincinnati Children's Hospital
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Myl1 | myosin, light polypeptide 1 |
These mice strongly express cre recombinase from the skeletal muscle-specific Myl1 (myosin, light polypeptide 1) promoter. When test crossed with an Erbb4 floxed strain, sequences were deleted selectively in skeletal muscle tissue, including gastrocnemius, tibialis anterior and biceps femoris muscles, and not in brain, liver, heart or stomach.
Cre, carrying a nuclear localization signal (nls), and a loxP-flanked PGK-neo cassette were inserted immediately upstream of the initiation ATG in exon 1 of the Myl1 (myosin, light polypeptide 1) gene. The mutation was created by homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. PGK-neo was removed by crossing resultant mice with deleter CMV-cre animals. This strain was maintained on a mixed genetic background incorporating 129, C57BL/6 and FVB by the donating laboratory.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Mice carrrying this allele express cre recombinase in skeletal muscle tissue, including gastrocnemius, tibialis anterior and biceps femoris muscles. |
Allele Name | targeted mutation 1, Steven J Burden |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | mlccre; Mlc1f-cre; MLC-Cre; Mlcf-cre |
Gene Symbol and Name | Myl1, myosin, light polypeptide 1 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Mice carrrying this allele express cre recombinase in skeletal muscle tissue, including gastrocnemius, tibialis anterior and biceps femoris muscles. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 1 |
Molecular Note | Insertion of an NLS-cre gene into exon 1, immediately upstream of the initiator ATG. An adjacent lox-P flanked neomycin cassette was removed by Cre mediated recombination in the final allele. |
Homozygotes and heterozygotes are viable and fertile.
When using the STOCK Myl1tm1(cre)Sjb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024713 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Myl1<tm1(cre)Sjb> |
Frozen Mouse Embryo | STOCK Myl1<tm1(cre)Sjb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Myl1<tm1(cre)Sjb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Myl1<tm1(cre)Sjb>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Myl1<tm1(cre)Sjb>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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