B6;129P2-Or8a1^{tm40(Gnas*)Mom}/MomJ

Stock number: 024642
Product name: M71-caGs-GFP
Research areas: Cell Biology Research, Developmental Biology Research, Research Tools, Sensorineural Research

Description

This allele creates tricistronic expression of the endogenous OR8A1 (formerly called OLFR151), a constitutively active GNAS, and a GFP fluorescent marker all from the Olfr151 locus. This permits assessment of the impact of increased GNAS activity on the maturation and function of olfactory sensory neurons expressing wild-type OR8A1.

Detailed description

This allele creates tricistronic expression of the endogenous OR8A1 (formerly called OLFR151), a constitutively active GNAS, and a GFP fluorescent marker all from the Or8a1 locus. This permits assessment of the impact of increased GNAS activity on the maturation and function of olfactory sensory neurons expressing wild-type OR8A1. Histology of homozygotes found 16 of 36 olfactory bulbs had a GFP+ lateral glomerulus in the correct location for an OR8A1 glomerulus, but in 20 of those 36 there was failure of the GFP+ axons to converse into a glomerulus. Additionally, compound heterozygotes between this allele and Or8a1^tm27Mom, which has tauRFP2 bicistronically expressed with Or8a1, show axon migration to the same location, but these form compartmentalized glomeruli instead of unifying into a single glomerulus, indicative of distinct identities.

Development

This allele was generated in 129P2-derived ES cells. The chimera was bred to C57BL/6, and the line was then maintained by sibling intercrossing. Sperm was cryopreserved from heterozygous males.

Allele

Symbol: Or8a1^{tm40(Gnas*)Mom}
Name: targeted mutation 40, Peter Mombaerts
MGI accession ID: MGI:5788006
Generation method: Targeted
Attributes: Reporter; Constitutively active; Inserted expressed sequence
Marker symbol: Or8a1
Marker name: olfactory receptor family 8 subfamily A member 1
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,; Gnas,GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus,mouse, laboratory
Site of expression: Olfactory lateral glomerulus.
Molecular note: An internal ribosome entry site followed by sequence encoding a constitutively active GNAS followed by a second internal ribosome entry site followed by GFP was all inserted just downstream of the endogenous Olfr151 coding sequence to give tricistronic expression of all three.