Cxcr2fl/fl floxed mice may be useful for studying the neutrophil granulocyte lineage and the migration of neutrophils during infection.
Richard M Ransohoff, Cleveland Clinic
Cxcr2fl/fl floxed mutant mice possess loxP sites flanking exon 2 of the chemokine (C-X-C motif) receptor 2 (Cxcr2) gene. Cxcr2 encodes a G-protein-coupled receptor which mediates neutrophil migration to sites of inflammation. CXCR2 also plays roles in development, tumorigenesis, and demyelination. Homozygotes are viable and fertile. These mice express CXCR2 on the surface of neutrophils in peripheral blood, bone marrow and spleen. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.
For example, when these mice are bred to B6.Cg-Tg(Mx1-cre)1Cgn/J mice (Stock No. 003556), poly(I:C)-induced cre activation results in impaired neutrophil migration in sterile peritonitis.
A targeting vector was designed to insert a loxP site upstream of exon 2, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 2 of the chemokine (C-X-C motif) receptor 2 (Cxcr2) gene. The construct was electroporated into C57BL/6NTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into 129/SvEv blastocysts and resulting chimeric mice were bred to C57BL/6J mice. These mice were bred to C57BL/6J mice for at least one more generation. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Richard Ransohoff|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Cxcr2, chemokine (C-X-C motif) receptor 2|
|Strain of Origin||C57BL/6NTac|
|Molecular Note||A loxP site was inserted upstream of exon 2. An FRT flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 2.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Cxcr2fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #024638 in your Materials and Methods section.