B6(SJL)-Rpl13a^tm1.1Mazu/J

Stock number: 024632
Product name: L13a^flox/flox
Research areas: Research Tools

Description

These L13a^flox/flox mutant mice possess loxP sites flanking exons 2 through 8 of the Rpl13a gene. This strain may be useful for generating conditional mutations in applications related to ribosomal protein L13a-dependent translational silencing.

Detailed description

These mice possess loxP sites on either side of exons 2 through 8 of the targeted Rpl13a gene. The protein encoded by the Rpl13a gene is a structural component of the 60S subunit of the ribosome and is also part of the gamma interferon-activated inhibitor of translation (GAIT) complex involved in inhibition of inflammatory interferon-gamma-induced transcript-selective translation. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-8 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in the myeloid cell lineage (see Stock No. 004781 for example), this mutant mouse strain may be useful in studies of translational control in macrophages.

Development

A loxP and FRT site flanked targeting vector containing a PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into unspecified C57BL/6 derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to transgenic mice (on the C57BL/6 genetic background) expressing FLPe recombinase under the control of the human ACTB promoter (Stock No. 005703). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Rpl13a^tm1.1Mazu
Name: targeted mutation 1.1, Barsanjit Mazumder
MGI accession ID: MGI:5502322
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Rpl13a
Marker name: ribosomal protein L13A
Chromosome: 7
Strain of origin: Not Specified
Molecular note: One loxP site was inserted in intron 1 and a neomycin resistance cassette flanked by both FRT sites and by loxP sites was inserted following exon8. Flp recombinase was then used to remove the selection cassette from properly targed mice. exons 2-8 were then flanked by loxP sites.