These transgenic mice constitutively express mouse Klf3 cDNA tagged with human c-myc under the regulatory control of the B-cell specific mouse Cd19 promoter. B cell development occurs normally, however B cell maturation in the spleen is heavily skewed towards the marginal zone (MZ) B cell subset.
Jorg Kirberg, Paul-Ehrlich-Institut
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Klf3 (Kruppel-like factor 3 (basic); also known as Bklf) promotes splenic marginal zone (MZ) B cell maturation. These transgenic mice constitutively express mouse Klf3 cDNA tagged with human c-myc under the regulatory control of the B-cell specific mouse Cd19 promoter. B cell development occurs normally, however B cell maturation in the spleen is heavily skewed towards the marginal zone (MZ) B cell subset where a 6- to 10-fold increase is seen. Whereas B-cell development in the bone marrow of KLF3-transgenic mice is unaffected, MZ B-cell numbers in spleen are increased considerably. This occurs cell autonomously, increasing both MZ and peritoneal B1 B-cell subsets.
Mouse Klf3 cDNA tagged with human c-myc at the N terminal end is driven by the mouse Cd19 promoter in this transgenic strain. The mutation was created and maintained on a C57BL/6 genetic background by the donating laboratory. Line 2 hemizygotes carry two copies of the transgene which may possibly be located on mouse Chromosome 17; the latter being based on observing linkage to Srf (serum response factor) by the donating laboratory.
Expressed Gene | Klf3, Kruppel-like factor 3 (basic), mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 2, Basel Institute for Immunology |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Cd19-Klf3)2Bii, transgene insertion 2, Basel Institute for Immunology |
Gene Synonym(s) | |
Promoter | Cd19, CD19 antigen, mouse, laboratory |
Expressed Gene | Klf3, Kruppel-like factor 3 (basic), mouse, laboratory |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | Mouse Klf3 cDNA tagged with human c-myc at the N terminal end is driven by the mouse Cd19 promoter. Mulitple lines were generated, however, data is from lines 2 and 23, which had indistinguishable phenotypes. Line 2 carries two copies of the transgene. This is a representative record for both lines. |
Hemizygotes are viable and fertile.
When using the C57BL/6-Tg(Cd19-Klf3)2Bii/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024539 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non CArrier for Tg(Cd19-Klf3)2Bii |
Frozen Mouse Embryo | C57BL/6-Tg(Cd19-Klf3)2Bii/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Cd19-Klf3)2Bii/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Cd19-Klf3)2Bii/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Cd19-Klf3)2Bii/J | $3373.50 |
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