C57BL/6-Tg(Cd19-Klf3)2Bii/J

Stock number: 024539
Research areas: Research Tools

Description

These transgenic mice constitutively express mouse Klf3 cDNA tagged with human c-myc under the regulatory control of the B-cell specific mouse Cd19 promoter. B cell development occurs normally, however B cell maturation in the spleen is heavily skewed towards the marginal zone (MZ) B cell subset.

Detailed description

Klf3 (Kruppel-like factor 3 (basic); also known as Bklf) promotes splenic marginal zone (MZ) B cell maturation. These transgenic mice constitutively express mouse Klf3 cDNA tagged with human c-myc under the regulatory control of the B-cell specific mouse Cd19 promoter. B cell development occurs normally, however B cell maturation in the spleen is heavily skewed towards the marginal zone (MZ) B cell subset where a 6- to 10-fold increase is seen. Whereas B-cell development in the bone marrow of KLF3-transgenic mice is unaffected, MZ B-cell numbers in spleen are increased considerably. This occurs cell autonomously, increasing both MZ and peritoneal B1 B-cell subsets.

Development

Mouse Klf3 cDNA tagged with human c-myc at the N terminal end is driven by the mouse Cd19 promoter in this transgenic strain. The mutation was created and maintained on a C57BL/6 genetic background by the donating laboratory. Line 2 hemizygotes carry two copies of the transgene which may possibly be located on mouse Chromosome 17; the latter being based on observing linkage to Srf (serum response factor) by the donating laboratory.

Allele

Symbol: Tg(Cd19-Klf3)2Bii
Name: transgene insertion 2, Basel Institute for Immunology
MGI accession ID: MGI:5558908
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Cd19-Klf3)2Bii
Marker name: transgene insertion 2, Basel Institute for Immunology
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Klf3,Kruppel-like factor 3 (basic),mouse, laboratory
Molecular note: Mouse Klf3 cDNA tagged with human c-myc at the N terminal end is driven by the mouse Cd19 promoter. Mulitple lines were generated, however, data is from lines 2 and 23, which had indistinguishable phenotypes. Line 2 carries two copies of the transgene. This is a representative record for both lines.