STOCK Trpc7^tm1.1Lbi/Mmjax

Stock number: 024534
Product name: TRPC7^-
Research areas: Cell Biology Research, Neurobiology Research, Sensorineural Research

Description

Trpc7 (transient receptor potential cation channel, c7) knockout mice may be useful for studying the function of diacylglycerol sensitive cation channels.

Detailed description

Trpc7 (transient receptor potential cation channel, c7) encodes a diacylglycerol sensitive cation channel. Homozygous knockout mice are viable and fertile. Intrinsically photosensitive retinal ganglion cells (ipRGCs) from null mice exhibit a slightly altered response to light intensities at two points in the middle of the dose response curve, but do not appear to significantly alter the light sensitivity of the melanopsin phototransduction pathway. This strain may be useful for studying the function of diacylglycerol sensitive cation channels.

Development

A targeting vector containing loxP-flanked neomycin resistance was inserted upstream of exon 5 and a loxP site was inserted downstream of exon 5. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were interbred and offspring were crossed to B6.Cg-Tg(Sox2-cre)1Amc/J mice to remove exon 5. Offspring were interbred to generate a homozygous colony that does not carry the Cre transgene. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Allele

Symbol: Trpc7^tm1.1Lbi
Name: targeted mutation 1.1, Lutz Birnbaumer
MGI accession ID: MGI:5296004
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Trpc7
Marker name: transient receptor potential cation channel, subfamily C, member 7
Chromosome: 13
Strain of origin: 129S6/SvEvTac
Molecular note: A floxed neo cassette was inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 5. Cre-mediated recombination removed the neo cassette and exon 5. The absence of transcripts with exon 5 was confirmed by RT-PCR.