B6(SJL)-Zbtb16^{tm1.1(EGFP/cre)Aben}/J

Stock number: 024529
Product name: PLZF^GFPcre
Research areas: Research Tools

Description

This PLZF^GFPcre reporter strain incorporates an IRES-EGFPcre fusion protein expressed from the mouse PLZF/Zbtb16 promoter. EGFP and Cre recombinase are expressed in the NKT cell lineage.

Detailed description

Zbtb16 (zinc finger and BTB domain containing 16) encodes transcription factor PLZF which directs the developmental acquisition of the innate effector program of NKT cells.

This reporter strain incorporates an IRES-EGFPcre fusion protein constitutively expressed from the mouse PLZF/Zbtb16 promoter. EGFP is selectively expressed in NKT cell lineage, with early developmental stages 1 and 2 showing higher levels than mature stage 3 cells. Fluorescence is not found in bone marrow common lymphoid precursor (CLP), T or B cells of heterozygous mice.

When combined with a ROSA26-floxstop-YFP fate mapping allele (e.g. Stock No. 006148), nearly all NKT cells express YFP, as expected, although approximately 35% of cells in all lymphoid and myeloid lineages are also labeled with YFP. This reflects some expression of PLZF prior to the hematopoietic stem cell (HSC) stage.

Development

An EGFP-cre fusion protein and FRT-flanked neomycin resistance cassette were introduced immediately after the Zbtb16 stop codon (in exon 7) via homologous recombination in C57BL/6J-derived embryonic stem (ES) cells. Knock-in mice were bred to ACTB-FLPe animals (see Stock No. 005703) to excise the neomycin cassette and create the PLZF^GFPcre allele. This strain was maintained on a C57BL/6 genetic background by the donating laboratory.

Allele

Symbol: Zbtb16^{tm1.1(EGFP/cre)Aben}
Name: targeted mutation 1.1, Albert Bendelac
MGI accession ID: MGI:5532503
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Zbtb16
Marker name: zinc finger and BTB domain containing 16
Chromosome: 9
Strain of origin: C57BL/6J
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP and Cre recombinase are expressed in the NKT cell lineage.
Molecular note: An IRES linked to an EGFP-cre fusion protein and an FRT-flanked neomycin resistance cassette were introduced immediately after the Zbtb16 (Plzf) stop codon (in exon 7) via homologous recombination in C57BL/6J-derived embryonic stem (ES) cells. Mice were bred to ACTB-FLPe animals to excise the neomycin cassette and create the PLZF allele.