129S-Gnai3^tm1Lbi Gnai1^tm1Lbi/Mmjax

Stock number: 024525
Product name: Gi1- Gi3-
Strain synonyms: Gi1 plus Gi3
Research areas: Developmental Biology Research, Internal/Organ Research

Description

Gnai1 and Gnai3 double knockout mice exhibit fusions of ribs and lumbar vertebrae. This strain may be useful for studying the role of the inhibitory heterotrimeric G proteins.

Detailed description

Gnai1 (guanine nucleotide binding protein (G protein), alpha inhibiting 1) or Gi1 and Gnai3 (guanine nucleotide binding protein (G protein), alpha inhibiting) or Gi3 encode the alpha 1 and 3 subunits of a G protein inhibitory complex. Mice homozygous for these two knockout alleles exhibit fusions of ribs and lumbar vertebrae. The fusion phenotype is more severe than in the homozygous Gnai3 knockout. This strain may be useful for studying the role of the inhibitory heterotrimeric G proteins.

Development

A targeting vector containing a neomycin resistance cassette was used to disrupt exon 3 of the Gnai1 gene. The construct was electroporated into 129S/SvEv-derived EK.CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice for an unknown number of generations.

A targeting vector containing a neomycin resistance cassette was used to disrupt part of intron 5 and exon 6 of the Gnai3 gene. The construct was electroporated into 129S/SvEv-derived EK.CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice for an unknown number of generations. The double mutant was generated by crossing the two strains together and breeding to produce double homozygotes.

Allele

Symbol: Gnai1^tm1Lbi
Name: targeted mutation 1, Lutz Birnbaumer
MGI accession ID: MGI:3803636
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Gnai1
Marker name: guanine nucleotide binding protein (G protein), alpha inhibiting 1
Chromosome: 5
Strain of origin: 129S/SvEv-Gpi1^c
Molecular note: A neo cassette was inserted into exon 3.

Allele

Symbol: Gnai3^tm1Lbi
Name: targeted mutation 1, Lutz Birnbaumer
MGI accession ID: MGI:3652743
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Gnai3
Marker name: guanine nucleotide binding protein (G protein), alpha inhibiting 3
Chromosome: 3
Strain of origin: 129S/SvEv-Gpi1^c
Molecular note: Part of intron 5 and exon 6 were replaced with a neo cassette.