Male mice homozygous for the null Mns1 or meiosis-specific nuclear structural 1 allele are infertile, exhibit a reduction in sperm production, immotile sperm and abnormal flagella. Some mice exhibit situs inversus, left isomerism and hydrocephalus. This strain may be useful for studying male infertility and primary ciliary dyskinesia (PCD).
Jeremy Wang, University of Pennsylvania
Mns1 or meiosis-specific nuclear structural 1 encodes a protein with long coiled-coil domains associated with spermiogenesis and motile ciliary functions. Homozygous null male mice are infertile. Sperm production is reduced to 8% of wild type and the remaining sperm are immotile. Abnormal assembly of sperm flagella results in disruption to the characteristic 9+2 microtubular axoneme arrangement and disorganized outer dense fibers. A percentage of homozygous mice exhibit situs inversus, left isomerism and hydrocephalus. Tracheal motile cilia have a normal 9+2 axonemal structure but lack some of the outer dynein arms. This strain may be useful for studying male infertility and primary ciliary dyskinesia (PCD).
A targeting vector containing neomycin resistance gene was used to disrupt exons 3 through 8. The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and maintained by sibling mating. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Jeremy Wang|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Mns1, meiosis-specific nuclear structural protein 1|
|Strain of Origin||(C57BL/6 x 129S4/SvJae)F1|
|Molecular Note||Exons 3 - 8 were replaced with a floxed neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in testes and trachea from homozygous mice.|
While maintaining a live colony, these mice are bred as heterozygotes or homozygote female x heterozygote male. Male mice homozygous for the mutation are sterile.
When using the B6;129S4-Mns1tm1Jw/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37095 in your Materials and Methods section.
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