Gata6 Tg mice express Gata6 cDNA under control of a tetracycline operator. When mated to a mutant strain expressing a tetracycline-controlled transactivator protein, expression of GATA6 may be regulated with doxycycline. These mice may be useful when studying embryonic axial patterning.
Andrew B. Lassar, Harvard Medical School
Gata6 Tg mice express GATA binding protein 6 (Gata6) cDNA under control of a tetracycline operator (tetO). Hemizygotes are viable and fertile. GATA6 is a zinc-finger-containing transcription factor involved in the regulation of early axial patterning, cellular differentiation, and organogenesis. Expressed is first seen at the eight-cell stage, and is predominately seen in forelimb buds and hindlimb buds in an anterior to posterior gradient in developing embryos. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of GATA6 protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring.
These Gata6 Tg mice contain a GATA binding protein 6 (Gata6) cDNA under control of a tetracycline operator (tetO). The construct was microinjected into fertilized oocytes, and founder mice were bred to C57BL/6 mice to establish a colony. These mice were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Expressed Gene||Gata6, GATA binding protein 6, mouse, laboratory|
|Site of Expression|
|Allele Name||transgene insertion 1, Andrew Lassar|
|Allele Type||Transgenic (Inducible, Inserted expressed sequence)|
|Gene Symbol and Name||Tg(tetO-Gata6)1Abl, transgene insertion 1, Andrew Lassar|
|Promoter||tetO, tet operator,|
|Expressed Gene||Gata6, GATA binding protein 6, mouse, laboratory|
|Strain of Origin||Not Specified|
|Molecular Note||The construct contains a GATA binding protein 6 (Gata6) cDNA under control of a tetracycline operator (tetO).|
When maintaining a live colony, transgenic mice may be bred to wildtype (noncarrier) mice from the colony or with C57BL/6J inbred mice (Stock No. 000664).
When using the STOCK Tg(tetO-Gata6)1Abl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024509 in your Materials and Methods section.
|Hemizygous or Non carrier for Tg(tetO-Gata6)1Abl|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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