Male mice homozygous for the null Golga3 or golgi autoantigen a3 allele (repro27) are sterile and exhibit significant germ cell loss due to apoptosis. Surviving spermatids exhibit multiple defects. The donating investigator reports that the phenotype is more severe on the C57BL/6J background. This strain may be useful for studying the role of golgin in male infertility.
Dr. Carol C Linder, NM Highlands University
Golga3 or golgi autoantigen a3 encodes a member of the golgin family of proteins associated with the Golgi complex and has regulatory roles in protein trafficking, apoptosis, Golgi complex positioning and spermatogenesis. The repro27 mutation is a premature stop codon that results in the absence of protein expression. Male mice homozygous for the mutation are sterile. Spermatogenesis is disrupted in late meiosis and significant germ cell loss occurs between 15-18 days post-partum due to apoptosis. Surviving spermatids exhibit defective acrosome formation, abnormal head and tail development and vacuolization in seminiferous epithelium. Spermatozoa are found in low concentrations with diminished motility. The donating investigator reports that the phenotype is more severe on the C57BL/6J background.
This strain may be useful for studying the role of golgin in male infertility.
This point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males at The Jackson Laboratory Reproductive Genomics Program. Mutagenized males were outcrossed to C3HeB/FeJ females. Progeny were backcrossed to C3HeB/FeJ for 10 generations and then maintained by intercross in the laboratory of Dr. Carol Linder at New Mexico Highlands University. The mutation results in a C to T transversion in exon 18 and alters a glutamine to a stop codon. Upon arrival, mice were bred to C3HeB/FeJ (Stock No. 000658) for at least 1 generation to establish the colony.
|Allele Name||reproductive mutant 27, JAX Reproductive Mutagenesis Program|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||Golga3, golgi autoantigen, golgin subfamily a, 3|
|Strain of Origin||C57BL/6J|
|Molecular Note||ENU mutagenesis induced a point mutation (C to T) in exon 18 that results in the amino acid substitution of a stop codon for glutamine. Western blot analysis confirmed the absence of protein expression in the testes, brain and heart.|
While maintaining a live colony, these mice are bred as homozygote female x heterozygote male. Homozygous male mice are sterile.
When using the C3Fe.B6-Golga3repro27/LincMmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37050 in your Materials and Methods section.