B6.129S1-Rel^tm1Ukl/J

Stock number: 024341
Product name: rel^fl/fl
Research areas: Research Tools

Description

These c-Rel^fl mutant mice possess loxP and FRT sites flanking exon 1 of the Rel gene. This strain may be useful for generating conditional mutations in applications related to the NF-kB signal transduction pathway. Cre-mediated recombination also results in expression of GFP (Green Fluorescent Protein) which can be used to trace Rel ablation.

Detailed description

The Rel (reticuloendotheliosis oncogene), also known as the proto-oncogene c-Rel, encodes for a member of the Rel/NF-kappaB family of transcription factors which are involved in cell growth and survival, stress responses and inflammation. These mice possess loxP sites on either side of exon 1 of the Rel gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted and GFP expression in the cre-expressing tissue(s). When crossed to mice that express Flp recombinase, the entire targeting construct, including exon 1, are deleted in the FLP expressing tissues.

When bred to a strain expressing Cre recombinase in B lymphocytes (see Stock No. 006785 for example), or to a strain expressing Cre recombinase in germinal center B lymphocytes (see Stock No. 010611 for example), this mutant mouse strain may be useful in studies of mature B-cell development and activation and germinal center B-cell differentation.

Development

A loxP site flanked targeting vector containing a PGKneo cassette was utilized in the construction of this mutant. Sequence encoding a promoterless eGFP minigene, a loxP site and the PGKneo cassette was inserted upstream of exon 1 of the targeted gene, and another loxP site followed by the PGK promoter was inserted downstream of exon 1. The entire targeted region was flanked by FRT sites. This construct was electroporated into 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resulting chimeric male animals were crossed to C57BL/6 females, and were then backcrossed to C57BL/6 for 15 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Rel^tm1Ukl
Name: targeted mutation 1, Ulf Klein
MGI accession ID: MGI:5568752
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Rel
Marker name: reticuloendotheliosis oncogene
Marker synonyms: c-Rel; HIVEN86A; IMD92
Chromosome: 11
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP will be expressed in cells where promoters driving Cre recombinase are expressed.
Molecular note: Sequence encoding a promoterless eGFP minigene, a loxP site and the PGKneo cassette was inserted upstream of exon 1 of the targeted gene, and another loxP site followed by the PGK promoter was inserted downstream of exon 1. The entire targeted region was flanked by FRT sites.