These NOD.TNFR1KO/TNFR2KO double mutant mice exhibit resistance to spontaneous type 1 diabetes and have applications in studies of type 1 diabetes.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tnfrsf1a | tumor necrosis factor receptor superfamily, member 1a |
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tnfrsf1b | tumor necrosis factor receptor superfamily, member 1b |
Mice that are homozygous for both targeted mutations are viable, fertile and
completely resistant to spontaneous type 1 diabetes (in mice observed up to 30 weeks). When activated diabetogenic CD4+ T cells from NOD.Cg-Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/DoiJ
(Stock No. 004460)
are transferred to these NOD.TNFR1KO/TNFR2KO double mutant mice, diabetes development is delayed.
Transfer of activated G9C8 clone cells (TCR from a CD8+ T cell clone specific for an insulin-derived peptide (InsB15-23) bound to Kd MHC class I molecule) to the NOD.TNFR1KO/TNFR2KO mice, results in delayed diabetes development and only 75% of the mice developing diabetes.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Allele Name | targeted mutation 1, Immunex Research and Development Corporation |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | p55-; p55 KO; Preschon Tnfrsf1a-; TNF-alpha-R1; TNFR-; TNFR1-; TNFRI-; Tnfrsf1atm1Imx |
Gene Symbol and Name | Tnfrsf1a, tumor necrosis factor receptor superfamily, member 1a |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 6 |
Molecular Note | A PGK-neomycin resistance cassette replaced 4kb of sequence, including exons 2 to 5 (amino acids 30 - 184). Lack of gene product was shown functionally. |
Mutations Made By | Dr. Jacques Peschon, Amgen |
Allele Name | targeted mutation 1, Immunex Research and Development Corporation |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | p75-; p75 KO; TNFbeta ko; TNFR2-; TNFRII-; Tnfrsf1btm1Imx |
Gene Symbol and Name | Tnfrsf1b, tumor necrosis factor receptor superfamily, member 1b |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 4 |
Molecular Note | A PGK-neomycin resistance cassette replaced 1kb of sequence encoding amino acids 3 - 26. Lack of gene product was shown functionally. |
Mutations Made By | Dr. Jacques Peschon, Amgen |
When maintaining a live colony, these mice can be bred homozygous for both alleles.
When using the NOD.Cg-Tnfrsf1btm1Imx Tnfrsf1atm1Imx/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024314 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tnfrsf1b<tm1Imx> , Heterozygous for Tnfrsf1a<tm1Imx> |
Frozen Mouse Embryo | NOD.Cg-Tnfrsf1b<tm1Imx> Tnfrsf1a<tm1Imx>/J | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Tnfrsf1b<tm1Imx> Tnfrsf1a<tm1Imx>/J | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Tnfrsf1b<tm1Imx> Tnfrsf1a<tm1Imx>/J | $3373.50 |
Frozen Mouse Embryo | NOD.Cg-Tnfrsf1b<tm1Imx> Tnfrsf1a<tm1Imx>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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