Fap knock-in/knock-out mice may be useful in studies of active tissue remodeling, wound/tissue repair, and epithelial carcinogenesis, and as a reporter (lacZ expression) for monitoring endogenous Fap expression.
Peter Seither, Boehringer Ingelheim Pharma KG
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Fap | fibroblast activation protein |
The Fap gene encodes a gelatinase (protease with collagenase activity), which is involved in fibroblast growth regulation and epithelial-mesenchymal interactions during development, wound/tissue repair, and epithelial carcinogenesis. These mice carry a knock in/knock out mutation of the Fap gene in which part of exons 4 and 5 were replaced with a lacZ-neo cassette. The mutation results in an in-frame fusion of exon 4 with thelacZ gene. No gene product (protein) is detected in homozygous embryos. FAP mediated dipeptidyl-peptidase activity os not detected in protein extracts from homozygous embryos. Mice that are homozygous for the targeted mutation are viable and fertile, with no overt phenotype. Beta-galactosidase activity is detected as early as 10.5 d.p.c. in homozygous embryos.
A targeting vector containing a lacZ-PGK-NEO cassette was used to disrupt part of exons 4 and 5. The construct was electroporated into 129P2/OlaHsd derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were bred to C57BL/6 females. The mice were backcrossed to C57BL/6 for an unknown number of generations. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | Beta-galactosidase activity is detected as early as 10.5 d.p.c. in homozygous embryos. |
Allele Name | targeted mutation 2, Andreas Schnapp |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Fap- lacZ |
Gene Symbol and Name | Fap, fibroblast activation protein |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | Beta-galactosidase activity is detected as early as 10.5 d.p.c. in homozygous embryos. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 2 |
Molecular Note | The gene was disrupted by replacement of a portion of exons 4 and 5 with a lacZ-neo cassette via homologous recombination. The gene targeting event results in an in-frame fusion of exon 4 with the lacZ gene. Protein extracts from homozygous mutant embryos did not exhibit enzyme activity. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129P2-Faptm2Schn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024288 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Fap<tm2Schn> |
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