B6;129P2-Fap^tm2Schn/J

Stock number: 024288
Research areas: Research Tools

Description

Fap knock-in/knock-out mice may be useful in studies of active tissue remodeling, wound/tissue repair, and epithelial carcinogenesis, and as a reporter (lacZ expression) for monitoring endogenous Fap expression.

Detailed description

The Fap gene encodes a gelatinase (protease with collagenase activity), which is involved in fibroblast growth regulation and epithelial-mesenchymal interactions during development, wound/tissue repair, and epithelial carcinogenesis. These mice carry a knock in/knock out mutation of the Fap gene in which part of exons 4 and 5 were replaced with a lacZ-neo cassette. The mutation results in an in-frame fusion of exon 4 with thelacZ gene. No gene product (protein) is detected in homozygous embryos. FAP mediated dipeptidyl-peptidase activity os not detected in protein extracts from homozygous embryos. Mice that are homozygous for the targeted mutation are viable and fertile, with no overt phenotype. Beta-galactosidase activity is detected as early as 10.5 d.p.c. in homozygous embryos.

Development

A targeting vector containing a lacZ-PGK-NEO cassette was used to disrupt part of exons 4 and 5. The construct was electroporated into 129P2/OlaHsd derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were bred to C57BL/6 females. The mice were backcrossed to C57BL/6 for an unknown number of generations. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Fap^tm2Schn
Name: targeted mutation 2, Andreas Schnapp
MGI accession ID: MGI:2656736
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Fap
Marker name: fibroblast activation protein
Chromosome: 2
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: Beta-galactosidase activity is detected as early as 10.5 d.p.c. in homozygous embryos.
Molecular note: The gene was disrupted by replacement of a portion of exons 4 and 5 with a lacZ-neo cassette via homologous recombination. The gene targeting event results in an in-frame fusion of exon 4 with the lacZ gene. Protein extracts from homozygous mutant embryos did not exhibit enzyme activity.