STOCK Tg(Tnnt2-cre)5Blh/JiaoJ

Stock number: 024240
Product name: cTnT-Cre
Strain synonyms: TNT-Cre
Research areas: Cardiovascular Research, Research Tools

Description

cTntCre mice express Cre recombinase under the control of the rat troponin T2, cardiac promoter. This strain represents an effective tool for generating early cardiomyocyte specific-targeted mutants that would be useful in studies of human congenital heart abnormalities.

Detailed description

cTntCre mice express Cre recombinase under the control of the rat troponin T2, cardiac (Tnnt2) promoter. Cre recombinase is active early in the cardiomyocyte lineage. It can induce recombination of a reporter gene in cardiac crescent staring from E7.5. Hemizygotes are viable and fertile. While hemizygous females do breed they have a 60% chance of being non-productive. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in cardiomyocytes.

For example, when bred to a strain expressing a floxed-Bmp4 (bone morphogenetic protein 4) gene, this mutant mouse strain may be useful for studying Atrioventricular Septal Defect (AVSD).

Development

A transgenic construct containing cre coding sequence under the control of the rat troponin T2, cardiac (Tnnt2) promoter was introduced into (B6D2)F2 donor oocytes. Mice from founder line 5, with the highest level of cre recombination activity, were bred to and further maintained on a mixed background including C57BL/6, DBA, and 129. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Tnnt2-cre)5Blh
Name: transgene insertion 5, Brigid L Hogan
MGI accession ID: MGI:2679081
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Tnnt2-cre)5Blh
Marker name: transgene insertion 5, Brigid L Hogan
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F2
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: When these mice are crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in cardiomyocytes.
Molecular note: Sequence encoding cre recombinase was adjoined to the rat troponin T2 promoter. The promoter drove expression of cre recombinase in the cardiomyocyte lineage beginning at E7.5. Expression was observed in all myocardium at E10.5.