cTntCre mice express Cre recombinase under the control of the rat troponin T2, cardiac promoter. This strain represents an effective tool for generating early cardiomyocyte specific-targeted mutants that would be useful in studies of human congenital heart abnormalities.
Kai Jiao, University of Alabama
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
cTntCre mice express Cre recombinase under the control of the rat troponin T2, cardiac (Tnnt2) promoter. Cre recombinase is active early
in the cardiomyocyte lineage. It can induce recombination of a reporter gene in
cardiac crescent staring from E7.5. Hemizygotes are viable and fertile. While hemizygous females do breed they have a 60% chance of being non-productive. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in cardiomyocytes.
For example, when bred to a strain expressing a floxed-Bmp4 (bone morphogenetic protein 4) gene, this mutant mouse strain may be useful for studying Atrioventricular Septal Defect (AVSD).
A transgenic construct containing cre coding sequence under the control of the rat troponin T2, cardiac (Tnnt2) promoter was introduced into (B6D2)F2 donor oocytes. Mice from founder line 5, with the highest level of cre recombination activity, were bred to and further maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | When these mice are crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in cardiomyocytes. |
Allele Name | transgene insertion 5, Brigid L Hogan |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | cTntCre; cTnt-Cre; Tnnt2-Cre; TNT-Cre |
Gene Symbol and Name | Tg(Tnnt2-cre)5Blh, transgene insertion 5, Brigid L Hogan |
Gene Synonym(s) | |
Promoter | Tnnt2, troponin T2, cardiac type, rat |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | When these mice are crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in cardiomyocytes. |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | Sequence encoding cre recombinase was adjoined to the rat troponin T2 promoter. The promoter drove expression of cre recombinase in the cardiomyocyte lineage beginning at E7.5. Expression was observed in all myocardium at E10.5. |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony. While hemizygous females do breed they have a 60% chance of being non-productive. The fertility of these mice is reduced when maintained on a pure C57BL/6 background.
When using the cTnT-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #024240 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Tnnt2-cre)5Blh |
Frozen Mouse Embryo | STOCK Tg(Tnnt2-cre)5Blh/JiaoJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Tnnt2-cre)5Blh/JiaoJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Tnnt2-cre)5Blh/JiaoJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Tnnt2-cre)5Blh/JiaoJ Frozen Embryo | $3373.50 |
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