These floxed stop-brachury mice are suitable for use as a model for chordoma. Until crossed with a cre strain, a floxed stop cassette blocks expression of the chimeric protein.
David Alcorta, Duke University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, No functional change) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Chordomas are malignant tumors that originate from remnants of the notochord, often arising at the base of the skull and along the spine. Duplication mutations of the human brachyury gene (T) have been identified in 4 unrelated families with chordomas. These targeted mutation mice carry a floxed STOP cassette followed by a murine brachyury (T) cDNA and IRES-GFP sequence inserted into the Gt(ROSA)26Sor locus. Cre mediated excision of the flox-STOP cassette results in expression of the chimeric protein.
Mice that are homozygous for the targeted mutation are viable and fertile.
A targeting vector containing a floxed STOP cassette followed by murine brachury (T) cDNA and IRES-EGFP under the control of a beta-actin promoter, was inserted into the endogenous Gt(ROSA)26Sor locus. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission by crossing to C57BL/6. Heterozygotes were intercrossed to obtain homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | T, brachyury, T-box transcription factor T, mouse, laboratory |
Site of Expression | When combined with Cre recombinase Cre-mediated recombination is observed in a wide variety of tissues. |
Allele Name | targeted mutation 1, David Alcorta |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence, No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | T, brachyury, T-box transcription factor T, mouse, laboratory |
Site of Expression | When combined with Cre recombinase Cre-mediated recombination is observed in a wide variety of tissues. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 6 |
Molecular Note | A targeting vector containing a floxed STOP cassette followed by murine brachury (T) cDNA and IRES-EGFP under the control of a beta-actin promoter, was inserted into the endogenous Gt(ROSA)26Sor locus. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the floxed stop-brachury mouse strain in a publication, please cite the originating article(s) and include JAX stock #024179 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm1(Actb-T,-GFP)Dalco> |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(Actb-T -GFP)Dalco>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(Actb-T -GFP)Dalco>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(Actb-T -GFP)Dalco>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Gt(ROSA)26Sor<tm1(Actb-T -GFP)Dalco>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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