B6;129-Gt(ROSA)26Sor^{tm1(Actb-T,-GFP)Dalco}/J

Stock number: 024179
Product name: floxed stop-brachury
Research areas: Cancer Research, Neurobiology Research, Research Tools

Description

These floxed stop-brachury mice are suitable for use as a model for chordoma. Until crossed with a cre strain, a floxed stop cassette blocks expression of the chimeric protein.

Detailed description

Chordomas are malignant tumors that originate from remnants of the notochord, often arising at the base of the skull and along the spine. Duplication mutations of the human brachyury gene (T) have been identified in 4 unrelated families with chordomas. These targeted mutation mice carry a floxed STOP cassette followed by a murine brachyury (T) cDNA and IRES-GFP sequence inserted into the Gt(ROSA)26Sor locus. Cre mediated excision of the flox-STOP cassette results in expression of the chimeric protein. Mice that are homozygous for the targeted mutation are viable and fertile.

Development

A targeting vector containing a floxed STOP cassette followed by murine brachury (T) cDNA and IRES-EGFP under the control of a beta-actin promoter, was inserted into the endogenous Gt(ROSA)26Sor locus. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission by crossing to C57BL/6. Heterozygotes were intercrossed to obtain homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Gt(ROSA)26Sor^{tm1(Actb-T,-GFP)Dalco}
Name: targeted mutation 1, David Alcorta
MGI accession ID: MGI:5543261
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Inserted expressed sequence; No functional change
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: GFP,Green Fluorescent Protein,; T,brachyury, T-box transcription factor T,mouse, laboratory
Site of expression: When combined with Cre recombinase Cre-mediated recombination is observed in a wide variety of tissues.
Molecular note: A targeting vector containing a floxed STOP cassette followed by murine brachury (T) cDNA and IRES-EGFP under the control of a beta-actin promoter, was inserted into the endogenous Gt(ROSA)26Sor locus.