B6;129S-Kdm6a^tm1.1Kaig/J

Stock number: 024177
Product name: UTX^flox
Research areas: Research Tools

Description

These UTX^flox mutant mice possess loxP sites flanking exon 24 of the Kdm6a gene. This strain may be useful for generating conditional mutations in applications related to development and histone methylation.

Detailed description

The Kdm6a gene encodes a histone demethylase that catalyzes the demethylation of tri/dimethylated lysine 27 of histone H3. These mice possess loxP sites on either side of exon 24 of the targeted gene. Homozygous females and hemizygous males (this gene is X linked) are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 24 deleted in the cre-expressing tissues.

When female mutant mice are bred to a strain with widespread Cre recombinase expression (see Stock No. 003724), or to a strain with Cre recombinase in spermatids (see Stock No. 003328), this mutant mouse strain may be useful in studies of development.

Development

A FRT site flanked targeting vector containing a NEO selection cassette and a 3' loxP site was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 24 of the targeted gene, and another loxP site was inserted upstream of exon 24. This construct was electroporated into (C57BL/6N x 129S6/SvEvTac)F1 derived iTL1 BA1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to knock in mice (on the 129S4 genetic background) expressing Flp recombinase driven by the Gt(ROSA)26Sor promoter (Stock No. 003946). Mice that retained the loxP site flanked exon 24 were then bred to remove the Flpe recombinase allele. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Kdm6a^tm1.1Kaig
Name: targeted mutation 1.1, Kai Ge
MGI accession ID: MGI:5448754
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Kdm6a
Marker name: lysine (K)-specific demethylase 6A
Chromosome: X
Strain of origin: Not Specified
Molecular note: A loxP site was inserted upstream of exon 24. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 24. Flp-mediated recombination removed the neo cassette inserted downstream of the modified exon 24. The modified exon 24 remains floxed.