These UTXflox mutant mice possess loxP sites flanking exon 24 of the Kdm6a gene. This strain may be useful for generating conditional mutations in applications related to development and histone methylation.
Kai Ge, National Institutes of Health, NIH
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Kdm6a | lysine (K)-specific demethylase 6A |
The Kdm6a gene encodes a histone demethylase that catalyzes the demethylation of tri/dimethylated lysine 27 of histone H3.
These mice possess loxP sites on either side of exon 24 of the targeted gene. Homozygous females and hemizygous males (this gene is X linked) are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 24 deleted in the cre-expressing tissues.
When female mutant mice are bred to a strain with widespread Cre recombinase expression (see Stock No. 003724), or to a strain with Cre recombinase in spermatids (see Stock No. 003328),
this mutant mouse strain may be useful in studies of development.
A FRT site flanked targeting vector containing a NEO selection cassette and a 3' loxP site was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 24 of the targeted gene, and another loxP site was inserted upstream of exon 24. This construct was electroporated into (C57BL/6N x 129S6/SvEvTac)F1 derived iTL1 BA1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to knock in mice (on the 129S4 genetic background) expressing Flp recombinase driven by the Gt(ROSA)26Sor promoter (Stock No. 003946). Mice that retained the loxP site flanked exon 24 were then bred to remove the Flpe recombinase allele.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1.1, Kai Ge |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | UTXflox |
Gene Symbol and Name | Kdm6a, lysine (K)-specific demethylase 6A |
Gene Synonym(s) | |
Strain of Origin | Not Specified |
Chromosome | X |
Molecular Note | A loxP site was inserted upstream of exon 24. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 24. Flp-mediated recombination removed the neo cassette inserted downstream of the modified exon 24. The modified exon 24 remains floxed. |
When maintaining a live colony, these mice can be bred as homozygous females and hemizygous males. The Kdm6a gene is X linked.
When using the UTXflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #024177 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X linked - Heterozygous female and Non carrier males |
Frozen Mouse Embryo | B6;129S-Kdm6a<tm1.1Kaig>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S-Kdm6a<tm1.1Kaig>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S-Kdm6a<tm1.1Kaig>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S-Kdm6a<tm1.1Kaig>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.