BXD78/2RwwJ

Stock number: 024029
Research areas: Research Tools

Description

The BXD#/Rww recombinant inbred (RI) strains originate from crosses between C57BL/6J (000664) females and DBA/2J (000671) males and were generated using a strategy of advanced intercrosses (AI). They may be used to study the genetics of behavioral phenotypes (including alcohol and drug addiction, stress, and locomotor activity) and complex or potentially complex physiologic phenotypes (including differences in organ weight and bone mineral density).

Detailed description

The BXD recombinant inbred (RI) strains are used to study the genetics of behavioral phenotypes including alcohol and drug addiction, stress, and locomotor activity. The BXD set of RI strains also are used in the genetic analysis of numerous complex or potentially complex physiologic phenotypes including differences in organ weight and bone mineral density.

The BXD#/Rww set was generated using a strategy of advanced intercrosses (AI). The AI technique produces recombinant RI strains which incorporate approximately twice as many recombinations as the standard RI strains. The addition of the AI BXD RI lines to the existing BXD set creates the largest of the mouse RI mapping panels. This set is useful in QTL mapping and analysis of gene function.

The strain distribution pattern (SDP) for the BXD RI strains is available through the Mouse Genome Informatics

Contributed Data Sets and Gene Network.

Additional tools and information are presented through the Mouse Phenome Database

Specialized Strain Panel Query Form, and

Gene Network .

Development

The BXD#/Rww advanced recombinant inbred (ARI) lines are derived from two independently derived advanced intercrosses between the C57BL/6J and DBA/2J progenitor strains generated at Princeton University and the University of Tennessee. Crosses at Princeton were initiated using B6D2F1/J mice crossed to generate B6D2F2 mice. These mice were randomly mated to generate a G3 population. Subsequent matings were chosen to minimize the number of common parents. AIL from the Tennessee crosses were initiated with reciprocal crosses of the parental strains. As a result, mice from the Tennessee set can have the Y chromosome and mitochondrial genome from either progenitor. Mice from the Princeton set have a Y chromosome from DBA/2J and the mitochondrial genome from C57BL/6J. All ARI lines were intercrossed for 9-14 generations before inbreeding. Like the Princeton crosses, the Tennessee crosses also sought to miminize common ancestors in each cross although fewer breeding cages per generation were used. The original line, BXD78/RwwJ, became extinct before it reached The Jackson Laboratory. This line, BXD78/2RwwJ, was generated by backcrossing a single BXD78 male to a C57BL/6 female through 5 backcrosses, and then sibling mating for 20 generations.

This line was donated to The Jackson Laboratory Repository in 2013 by Dr. Robert Williams.