Ngn3-Cre mice express Cre Recombinase in all endocrine cell types in the pancreatic islet. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked gene in neurogenin 3 expressing tissues.
Dr. S. Brian Wilson, University of Florida
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Hemizygous Ngn3-Cre mice are viable and fertile. Expression of the transgene is directed by a neurogenin 3 (Neurog3) promoter in all endocrine cell types in the pancreatic islet. When bred with a mouse containing a loxP site-flanked sequence of interest, cre-mediated recombination results in deletion of the flanked gene in islets.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct was designed containing Cre Recombinase under the control of the neurogenin 3, Neurog3, promoter, was introduced into B6CBAF1 donor eggs. Founder lines were subsequently established. The transgenic mice were crossed to outbred CD-1 mice. Dr. Brian Wilson from University of Florida bred these mice to NOD/ShiLtJ mice for at least 22 generations. Mice were sent to The Jackson Laboratory as Stock No. 023973. Upon arrival, mice were bred to NOD/ShiLtJ inbred mice (Stock No. 001976) for at least one generation to establish a colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase is expressed in all endocrine cell types in the pancreatic islet |
Allele Name | transgene insertion 1, Douglas A Melton |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Ngn3-Cre |
Gene Symbol and Name | Tg(Neurog3-cre)1Dam, transgene insertion 1, Douglas A Melton |
Gene Synonym(s) | |
Promoter | Neurog3, neurogenin 3, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase is expressed in all endocrine cell types in the pancreatic islet |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
Molecular Note | Expression of Cre recombinase is driven by the 6.5 kb upstream sequence from the mouse neurogenin 3 gene. |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make this strain homozygous.
When using the Ngn3-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #023973 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizyous or Non Carrir for Tg(Neurog3-cre)1Dam |
Frozen Mouse Embryo | NOD.Cg-Tg(Neurog3-cre)1Dam/SbwJ | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Tg(Neurog3-cre)1Dam/SbwJ | $2595.00 |
Frozen Mouse Embryo | NOD.Cg-Tg(Neurog3-cre)1Dam/SbwJ | $3373.50 |
Frozen Mouse Embryo | NOD.Cg-Tg(Neurog3-cre)1Dam/SbwJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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