These Fgf3c mice have loxP sites flanking exon2, and may be useful for studying the development of the head including inner and ears, and teeth.
Suzanne Mansour, University of Utah
These Fgf3c mice possess loxP sites flanking exon 2 of the fibroblast growth factor 3 (Fgf3) gene. The donating investigator reports that a tetracycline response element present upstream of exon 2 has no effect on expression unless a tetO binding protein is present and activated. FGF3 is expressed in the hindbrain, pharyngeal endoderm and tailbud, and is involved in cell division, embryonic development, regulation of cell growth and maturation, formation of blood vessels, and wound healing. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.
For example, when bred to BALB/c-Tg(CMV-cre)1Cgn/J mice (Stock No. 003465), FGF3 null mice fail to initiate inner ear and limb development.
A targeting vector was designed to insert a tetracycline response element, a single loxP site, and a frt-flanked neomycin (neo) and kanamycin (kan) resistance cassette upstream of exon 2 of the fibroblast growth factor 3 (Fgf3) gene. A second loxP site was inserted downstream of exon 2. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred to C57BL/6 mice. Offspring were bred with
129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J transgenic mice (Stock No. 003946) to delete the selection cassette. Progeny were crossed to remove the Flp-expressing transgene. These mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1.2, Suzanne L Mansour|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Fgf3, fibroblast growth factor 3|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Flp mediated recombination removed the neo cassette leaving exon 2 floxed.|
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129-Fgf3tm1.2Sms/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023900 in your Materials and Methods section.