B6;129-Gt(ROSA)26Sor^tm6(Fzd6)Nat/J

Stock number: 023843
Product name: R26-LSL-3xHA-Fz6
Research areas: Developmental Biology Research, Research Tools

Description

This knock-in line at the Gt(ROSA)26Sor (gene trap ROSA 26, Philippe Soriano) locus gives no expression of triple hemagglutinin (HA) epitope-tagged Fzd6 (frizzled homolog 6 (Drosophila)) in the absence of cre-mediated recombination. Following cre-mediated recombination, a loxP-stop-loxP cassette is excised and the downstream triple HA epitope-tagged Fzd6 coding region is expressed, driven by the Gt(ROSA)26Sor promoter.

Detailed description

This knock-in line at the Gt(ROSA)26Sor (gene trap ROSA 26, Philippe Soriano) locus gives no expression of triple hemagglutinin (HA) epitope-tagged Fzd6 (frizzled homolog 6 (Drosophila)) in the absence of cre-mediated recombination. Following cre-mediated recombination, a loxP-stop-loxP cassette is excised and the downstream triple HA epitope-tagged Fzd6 coding region is expressed, driven by the Gt(ROSA)26Sor promoter. Following cre-recombination, the triple HA epitope-tagged Fzd6 can rescue the Fzd6 knockout hair patterning phenotype.

Development

The following elements were inserted, in the following order, into the Gt(ROSA)26Sor locus by homologous recombination in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells: loxP-three stop transcription signals-loxP, a Fzd6 coding region with a triple hemagglutinin (HA) tag inserted immediately C-terminal to the signal peptide. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating lab.

Allele

Symbol: Gt(ROSA)26Sor^tm6(Fzd6)Nat
Name: targeted mutation 6, Jeremy Nathans
MGI accession ID: MGI:5510965
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); Inserted expressed sequence; No functional change
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: Fzd6,frizzled class receptor 6,mouse, laboratory; HA tag,HA tag,
Molecular note: The following elements were inserted, in the following order, into the locus by homologous recombination: loxP-three stop transcription signals-loxP, a Fzd6 coding region with a triple hemagglutinin (HA) tag inserted immediately C-terminal to the signal peptide.