These mice carry a targeted knockout of Dazl (deleted in azoospermia-like) on the C57BL/6N genetic background. Homozygotes are infertile, but otherwise healthy. Germ cells fail to activate genes required for both male-specific and female-specific sexual differentiation in homozygous knockout mice. This phenotype appears to be sensitive to the genetic background.
David Page, Whitehead Institute for Biomedical Research
Dazl (deleted in azoospermia-like) is a germ cell-specific RNA binding protein that is expressed in post-migratory primordial germ cells as well as postnatal germ cells. On the C57BL/6N genetic background, it acts as a competence factor for gametogenesis.
These mice carry a targeted knockout of Dazl on the C57BL/6N genetic background. RT-PCR confirms the absence of transcript and western blot/immunohistochemistry fail to detect any protein generated from the null allele. Heterozygotes are viable and fertile. Homozygotes are infertile, but otherwise healthy. Germ cells fail to activate genes required for both male-specific and female-specific sexual differentiation in homozygous knockout mice. Post migratory germ cells retain PGC-like gene expression and eventually die. This phenotype appears to be sensitive to strain background, however.
Exons 6 and 7, and all but five amino acids of exon 5 were replaced by a neomycin resistance cassette via homologous recombination in 129P2/OlaHsd-derived CGR8 embryonic stem (ES) cells. This strain was originally maintained on an outbred MF1 genetic background in Howard Cooke's laboratory, but has since been backcrossed to C57BL/6NTac by the donating laboratory for more than 27 generations. Upon arrival at The Jackson Laboratory, this line was re-derived with C57BL/6NJ (see Stock No. 005304). All Y chromosomes and mitochondria are of C57BL/6 origin.
|Allele Name||targeted mutation 1, Howard J Cooke|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Dazl-; dazlTm1hgu; DazlaTm1Hgu; Dazlko|
|Gene Symbol and Name||Dazl, deleted in azoospermia-like|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Exons 5 through 7 were disrupted by the insertion of a neomycin selection cassette. Mutant transcripts were undetected via RT-PCR analysis of total RNA isolated from heterozygous mice. Western blot analysis of testis extracts showed an absence of protein in homozygous mutant mice.|
Heterozygotes are viable and fertile. Some of the males are poor breeders. Homozygotes are infertile, but otherwise healthy.
When using the B6N.129P2-Dazltm1Hjc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023802 in your Materials and Methods section.