B6;FVB-Tg(Aldh1l1-cre)JD1884Htz/J

Stock number: 023748
Research areas: Neurobiology Research, Research Tools

Description

This transgenic strain expresses cre recombinase from the mouse Aldh1l1 (aldehyde dehydrogenase 1 family, member L1) promoter. This strain enables tissue-specific excision of floxed genomic segments in astrocytes as well as some oligodendrocytes and neurons.

Detailed description

Expression of Aldh1l1 (aldehyde dehydrogenase 1 family, member L1) begins in spinal cord radial glia at the time of their commitment to gliogenesis. This transgenic strain expresses Cre from the mouse Aldh1l1 promoter. When crossed with a Gt(ROSA)26Sor-YFP floxed reporter strain, glial cells of the spinal cord exhibit fluorescence. Seventy-five percent of labeled cells also express GFAP (glial fibrillary acidic protein; a marker for astrocytes) and 15% co-express OLIG2 (oligodendrocyte transcription factor 2; a marker for oligodendrocytes). Only 10% express RBFOX3 (RNA binding protein, fox-1 homolog (C. elegans) 3; a marker for neurons, also called NeuN).

Development

Cre recombinase (Cre-I-Cre polyA), was inserted into the initiating ATG codon/first coding exon of Aldh1l1 in the C57BL/6J mouse BAC RP23-7M9. Purified BAC DNA was injected into FVB/N embryos. Resultant mice were crossed to C57BL/6J for a few generations by the donating laboratory. This is founder line JD1884.

Allele

Symbol: Tg(Aldh1l1-cre)JD1884Htz
Name: transgene insertion JD188, Nathaniel Heintz
MGI accession ID: MGI:5514272
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Aldh1l1-cre)JD1884Htz
Marker name: transgene insertion JD188, Nathaniel Heintz
Chromosome: UN
Strain of origin: FVB/NTac
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Glial cells of the spinal cord.
Molecular note: Cre recombinase (Cre-I-cre polyA),was inserted into the initiating ATG codon/first coding exon of Aldh1l1 in the C57BL/6J mouse BAC RP23-7M9. Purified BAC DNA was injected into FVB/N embryos. This is founder line JD1884. Resultant mice were crossed to C57BL/6J for a few generations.