This transgenic strain expresses cre recombinase from the mouse Aldh1l1 (aldehyde dehydrogenase 1 family, member L1) promoter. This strain enables tissue-specific excision of floxed genomic segments in astrocytes as well as some oligodendrocytes and neurons.
Nathaniel Heintz, The Rockefeller University
Genetic Background | Generation |
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?+pN1F16
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
Expression of Aldh1l1 (aldehyde dehydrogenase 1 family, member L1) begins in spinal cord radial glia at the time of their commitment to gliogenesis.
This transgenic strain expresses Cre from the mouse Aldh1l1 promoter. When crossed with a Gt(ROSA)26Sor-YFP floxed reporter strain, glial cells of the spinal cord exhibit fluorescence. Seventy-five percent of labeled cells also express GFAP (glial fibrillary acidic protein; a marker for astrocytes) and 15% co-express OLIG2 (oligodendrocyte transcription factor 2; a marker for oligodendrocytes). Only 10% express RBFOX3 (RNA binding protein, fox-1 homolog (C. elegans) 3; a marker for neurons, also called NeuN).
Cre recombinase (Cre-I-Cre polyA), was inserted into the initiating ATG codon/first coding exon of Aldh1l1 in the C57BL/6J mouse BAC RP23-7M9. Purified BAC DNA was injected into FVB/N embryos. Resultant mice were crossed to C57BL/6J for a few generations by the donating laboratory. This is founder line JD1884.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Glial cells of the spinal cord. |
Allele Name | transgene insertion JD188, Nathaniel Heintz |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Aldh1l1-cre)JD1884Htz, transgene insertion JD188, Nathaniel Heintz |
Gene Synonym(s) | |
Promoter | Aldh1l1, aldehyde dehydrogenase 1 family, member L1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Glial cells of the spinal cord. |
Strain of Origin | FVB/NTac |
Chromosome | UN |
Molecular Note | Cre recombinase (Cre-I-cre polyA),was inserted into the initiating ATG codon/first coding exon of Aldh1l1 in the C57BL/6J mouse BAC RP23-7M9. Purified BAC DNA was injected into FVB/N embryos. This is founder line JD1884. Resultant mice were crossed to C57BL/6J for a few generations. |
Hemizygotes are viable and fertile.
When using the B6;FVB-Tg(Aldh1l1-cre)JD1884Htz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023748 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizyous or Non Carrier for Tg(Aldh1l1-cre)JD1884Gsat/ |
Frozen Mouse Embryo | B6;FVB-Tg(Aldh1l1-cre)JD1884Htz/J | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Aldh1l1-cre)JD1884Htz/J | $2595.00 |
Frozen Mouse Embryo | B6;FVB-Tg(Aldh1l1-cre)JD1884Htz/J | $3373.50 |
Frozen Mouse Embryo | B6;FVB-Tg(Aldh1l1-cre)JD1884Htz/J | $3373.50 |
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