STOCK Nkx6-1^tm1.1Msan/J

Stock number: 023731
Product name: Nkx6.1
Research areas: Developmental Biology Research, Endocrine Deficiency Research, Research Tools

Description

Nkx6.1^flox mice may be useful for studying the development and maintenance of islet beta cells.

Detailed description

These Nkx6.1^flox mutant mice possess loxP sites flanking exon 2 of the NK6 homeobox 1 (Nkx6-1) gene. NKX6.1 is a transcription factor required for the development and maintenance of pancreatic beta cells. Homozygotes are viable and fertile. When bred to mice that express a tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in cre-expressing tissues of the offspring.

Development

A targeting vector was designed to insert a loxP site upstream of exon 2, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 2 of the NK6 homeobox 1 (Nkx6-1) gene. The construct was electroporated into 129S6/SvEvTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred with C57BL/6NCrl females. Offspring were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J (Stock No. 005703) transgenic mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. The donating investigator reported that these mice were bred to C57BL/6NCrl mice for at least 6 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Seven of the 27 markers throughout the genome were segregating with 129 suggesting an incomplete backcross.

Allele

Symbol: Nkx6-1^tm1.1Msan
Name: targeted mutation 1.1, Maike Sander
MGI accession ID: MGI:5501129
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Nkx6-1
Marker name: NK6 homeobox 1
Chromosome: 5
Strain of origin: 129S6/SvEvTac
Molecular note: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance (neo) cassette with a 3' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the neo cassette and left exon 2 floxed.