These Rimbp2flox mice may be useful for generating tissue specific deletions of RIMS binding protein 2.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Rimbp2 | RIMS binding protein 2 |
Rimbp2flox mutant mice possess loxP sites flanking exons 10 of the RIMS binding protein 2 (Rimbp2) gene. The frt-flanked antibiotic resistance cassettes are still present in these mice. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 10 deleted in the cre-expressing tissues.
A targeting vector was designed to insert a loxP site upstream of exon 10 followed by frt-flanked neomycin (neo) and puromycin (puro) resistance cassettes, and a second loxP site downstream of exon 10 of the RIMS binding protein 2 (Rimbp2) gene. The construct was electroporated into C57BL/6NTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to CD1 mice. These mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Thomas C Sudhof |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | B2N; Rimbp2flox |
Gene Symbol and Name | Rimbp2, RIMS binding protein 2 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6NTac |
Chromosome | 5 |
Molecular Note | A targeting vector was designed to insert a loxP site upstream of exon 10 followed by frt-flanked neomycin (neo) and puromycin (puro) resistance cassettes, and a second loxP site downstream of exon 10. |
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Rimbp2tm1Sud/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023644 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Rimbp2<tm1Sud> |
Frozen Mouse Embryo | STOCK Rimbp2<tm1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Rimbp2<tm1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Rimbp2<tm1Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Rimbp2<tm1Sud>/J Frozen Embryo | $3373.50 |
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