STOCK Rimbp2^tm1Sud/J

Stock number: 023644
Research areas: Research Tools

Description

These Rimbp2^flox mice may be useful for generating tissue specific deletions of RIMS binding protein 2.

Detailed description

Rimbp2^flox mutant mice possess loxP sites flanking exons 10 of the RIMS binding protein 2 (Rimbp2) gene. The frt-flanked antibiotic resistance cassettes are still present in these mice. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 10 deleted in the cre-expressing tissues.

Development

A targeting vector was designed to insert a loxP site upstream of exon 10 followed by frt-flanked neomycin (neo) and puromycin (puro) resistance cassettes, and a second loxP site downstream of exon 10 of the RIMS binding protein 2 (Rimbp2) gene. The construct was electroporated into C57BL/6NTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to CD1 mice. These mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.

Allele

Symbol: Rimbp2^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:5509348
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: B2N; Rimbp2flox
Marker symbol: Rimbp2
Marker name: RIMS binding protein 2
Marker synonyms: A930033C01Rik; mKIAA0318; PPP1R133; RBP2; RIM-BP2
Chromosome: 5
Strain of origin: C57BL/6NTac
Molecular note: A targeting vector was designed to insert a loxP site upstream of exon 10 followed by frt-flanked neomycin (neo) and puromycin (puro) resistance cassettes, and a second loxP site downstream of exon 10.