B6.129(FVB)-Cd68^tm1.1Shnd/J

Stock number: 023543
Product name: CD68^-
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These CD68 KO mice may be useful for studying the role of CD68 as a scavenger receptor during inflammatory responses and antigen presentation.

Detailed description

In this strain, a rtTA (reverse tetracycline regulated transactivator) was placed downstream of the transcriptional start site of the CD68 antigen (Cd68) gene, abolishing gene function. The donating investigator was not able to detect rtTA expression in these mice. CD68, also known as macrosialin, is a lysosome associated membrane protein which serves as a macrophage marker in bone marrow, brain, liver, and osteoclasts. It is upregulated by various inflammatory stimuli, including oxidized Low Density Lipoprotein. It may serve as a scavenger receptor. Homozygotes are viable and fertile. Dendritic cells from these mice exhibit enhanced antigen presenting activity when stimulated with ovalbumin. Macrophages also show increased lipid uptake.

Development

A targeting vector was designed to place an rtTA (reverse tetracycline regulated transactivator) and a loxP-flanked neomycin resistance (neo) cassette downstream of the transcriptional start site of the CD68 antigen (Cd68) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6J females. These mice were bred to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J mice (Stock No. 003724) to delete the neo cassette and the resulting offspring were bred together to remove the cre-expressing transgene. Progeny were bred to C57BL/6J mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Cd68^tm1.1Shnd
Name: targeted mutation 1.1, Christian Schindler
MGI accession ID: MGI:5506645
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd68
Marker name: CD68 antigen
Marker synonyms: GP110; Lamp4; macrosialin; Scard1
Chromosome: 11
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was designed to place an rtTA (reverse tetracycline regulated transactivator) and a loxP-flanked neomycin resistance (neo) cassette downstream of the transcriptional start site. Correctly targeted mice were bred to cre expressing mice to delete the neo cassette and the resulting offspring were bred together to remove the cre-expressing transgene.