B6.129P3-Rasgrp3^tm1Jstn/TbwnJ

Stock number: 023496
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

These RasGRP3^- mice may be useful when studying thymocyte development and maturation.

Detailed description

In these mice, a neomycin (neo) resistance gene interrupts exon 2 of the RAS guanyl releasing protein 3 (Rasgrp3) gene, abolishing gene expression. RasGRP3 is a guanine nucleotide-releasing factor needed for activation of the Ras-Erk1/2 pathway. RasGRP1 plays an important role in development, homeostasis and differentiation of thymocytes. Homozygous mice are viable and fertile. They exhibit mild B cell signaling defects and phenotypes.

Development

A targeting vector was designed to insert a neomycin (neo) resistance gene into exon 2 of the RAS guanyl releasing protein 3 (Rasgrp3) gene. The construct was electroporated into 129P3/J-derived embryonic stem (ES) cells and correctly targeted clones were injected into C57BL/6J blastocysts. Male chimeric mice were mated with C57BL/6J females to produce heterozygotes, offspring were mated to produce homozygotes. They were bred to C57BL/6J mice for at least 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Rasgrp3^tm1Jstn
Name: targeted mutation 1, James C Stone
MGI accession ID: MGI:3625862
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Rasgrp3
Marker name: RAS, guanyl releasing protein 3
Chromosome: 17
Strain of origin: 129P3/J
Molecular note: A neomycin resistance cassette was inserted into exon 2, interrupting the coding sequence downstream of codon 40 in the Ras exchange motif of the catalytic region.
General note: ES cell line = NX1.