Exons 2 and 3 of the Dpf1 (also known as BAF45b) gene have been deleted in this knockout mutant allele. Homozygous have no overt phenotype.
Gerald R Crabtree, Stanford University Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Dpf1 | D4, zinc and double PHD fingers family 1 |
Exons 2 and 3 of the Dpf1 (D4, zinc and double PHD fingers family 1; also known as BAF45b) gene have been deleted in this knockout mutant allele. Homozygous have no overt phenotype.
A loxP-flanked neomycin cassette was introduced to intron 1 and a loxP site was placed in intron 3 through homologous recombination in 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Exons 2 and 3 were excised with a cre recombinase on a C57BL/6 genetic background. This strain was backcrossed to 129S6/SvEvTac for an unknown number of times.
Allele Name | targeted mutation 1.1, Gerald R Crabtree |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Dpf1, D4, zinc and double PHD fingers family 1 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 7 |
Molecular Note | A loxP-flanked neomycin resistance cassette was introduced to intron 1 and a loxP site was placed in intron 3 through homologous recombination. Cre-mediated recombination removed the neomycin resistance cassette and exons 2 and 3. |
Heterozygotes are viable and fertile. Homozygotes are viable, but reportedly may not breed well.
When using the STOCK Dpf1tm1.1Grc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023427 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Dpf1<tm1.1Grc> |
Frozen Mouse Embryo | STOCK Dpf1<tm1.1Grc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Dpf1<tm1.1Grc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Dpf1<tm1.1Grc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Dpf1<tm1.1Grc>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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