STOCK Dpf1^tm1.1Grc/J

Stock number: 023427
Research areas: Developmental Biology Research

Description

Exons 2 and 3 of the Dpf1 (also known as BAF45b) gene have been deleted in this knockout mutant allele. Homozygous have no overt phenotype.

Detailed description

Exons 2 and 3 of the Dpf1 (D4, zinc and double PHD fingers family 1; also known as BAF45b) gene have been deleted in this knockout mutant allele. Homozygous have no overt phenotype.

Development

A loxP-flanked neomycin cassette was introduced to intron 1 and a loxP site was placed in intron 3 through homologous recombination in 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Exons 2 and 3 were excised with a cre recombinase on a C57BL/6 genetic background. This strain was backcrossed to 129S6/SvEvTac for an unknown number of times.

Allele

Symbol: Dpf1^tm1.1Grc
Name: targeted mutation 1.1, Gerald R Crabtree
MGI accession ID: MGI:5504408
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Dpf1
Marker name: D4, zinc and double PHD fingers family 1
Chromosome: 7
Strain of origin: 129S6/SvEvTac
Molecular note: A loxP-flanked neomycin resistance cassette was introduced to intron 1 and a loxP site was placed in intron 3 through homologous recombination. Cre-mediated recombination removed the neomycin resistance cassette and exons 2 and 3.