These floxed mutant mice possess loxP sites flanking the Sp8 coding region. They may be useful in applications related to the study of olfactory neurogenesis, and limb and craniofacial development.
Steven Potter, Children's Hospital Research Foundation
These Sp8flox mutant mice possess loxP sites flanking the coding region of the trans-acting transcription factor 8 (Sp8) gene. SP8 is a transcription factor that plays a role in embryonic limb development, craniofacial development, and olfactory neurogenesis. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have the entire coding region deleted in the cre-expressing tissues.
For example, when bred to mice having germ-line Cre expression, resulting homozygous mice exhibit defects of both forelimbs and hindlimbs, tail abnormalities, defects in anterior and posterior neuropore, exencephaly and spina bifida.
A targeting vector was designed to insert a loxP site upstream of exon 3 followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of the 3'UTR of the trans-acting transcription factor 8 (Sp8) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to Black Swiss mice. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 2, Sheila M Bell|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Sp8, trans-acting transcription factor 8|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||An FRT flanked neomycin selection cassette and a 5' loxP site were inserted in intron 2. A second loxP site was placed downstream of the zinc finger domain and stop codon in the 3' UTR. The coding sequence of the gene was thus surrounded by loxP sites. Sp8tm1Smb was derived from this allele.|
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Sp8tm2Smb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023415 in your Materials and Methods section.