These floxed-AP-2gamma mutant mice possess loxP sites flanking exon 6 of the transcription factor AP-2, gamma gene. This strain may be useful for studying embryogenesis.
Trevor Williams, University of Colorado, Denver
These floxed-AP-2gamma mutant mice possess loxP sites flanking exon 6 of the transcription factor AP-2, gamma (Tfap2c) gene. AP-2gamma is a transcription factor expressed early in embryogenesis in trophoblast cell lineage. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 6 deleted in the cre-expressing tissues.
A targeting vector was designed to insert a loxP site upstream of exon 6 of the transcription factor AP-2, gamma (Tfap2c) gene. A frt-flanked neomycin resistance (neo) cassette, followed by a second loxP site were placed downstream of exon 6. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ-7 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a actb-Flpe expression plasmid to delete the neo cassette. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred to Black Swiss mice. These mice were subsequently crossed to mice carrying the Tfap2atm2Will allele on a mixed Swiss Black x 129S background. Upon arrival, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony. The Tfap2atm2Will allele was bred out of this colony, and is maintained as Stock No. 023406.
|Allele Name||targeted mutation 2, Trevor Williams|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||AP-2gammalox; Gamflox|
|Gene Symbol and Name||Tfap2c, transcription factor AP-2, gamma|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||Exon 6 was flanked with loxP sites.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Tcfap2c flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #023408 in your Materials and Methods section.