Floxed-AP-2alpha mutant mice possess loxP sites flanking exons 5-6 of the transcription factor AP-2, alpha (Tfap2a) gene. This strain may be useful for studying the developing neural tube.
Trevor Williams, University of Colorado, Denver
These floxed-AP-2alpha mutant mice possess loxP sites flanking exons 5-6 of the transcription factor AP-2α gene. AP-2alpha is expressed in the neural tube, and in cranial and cardiac neural crest cells in the developing embryo, as well as in the progress zone of the limb bud, the developing kidney, the eye, and surface ectoderm. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 5-6 deleted in the cre-expressing tissues.
For example, when bred to B6.Cg-Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J mice (Stock No. 009107) expressing Cre recombinase in the developing neural tube, offspring are perinatal lethal due neural tube closure defects and cleft secondary palate. Some mutant mice can survive into adulthood and exhibit retarded craniofacial growth, abnormal middle ear development, and defects in pigmentation.
When bred to FVB-HhatTg(TFAP2A-cre)1Will/J mice (Stock No. 023407) expressing Cre recombinase in neural tube, and in cranial and cardiac neural crest cells in the developing embryo, offspring offspring exhibit a combination of midfacial growth anomalies.
A targeting vector was designed to insert a loxP site upstream of exon 5 followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 6 of the transcription factor AP-2, alpha gene (Tfap2a). The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ-7 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a actb-Flpe expression plasmid to delete the neo cassette. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were crossed to Black Swiss mice. These mice were subsequently crossed to mice carrying the Tfap2ctm2Will allele on a mixed Swiss Black x 129S background. Upon arrival, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony. The Tfap2ctm2Will allele was bred out of this colony, and is maintained as Stock No. 023408.
|Allele Name||targeted mutation 2, Trevor Williams|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Alflox; AP-2alphalox|
|Gene Symbol and Name||Tfap2a, transcription factor AP-2, alpha|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||Exons 5 and 6 were flanked by single loxP sites in introns 4 and 6. A single frt site remained immediately upstream of the 3' loxP site after the excision of an frt-flanked neo cassette used for selection.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Alflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #023406 in your Materials and Methods section.