Overview

Also Known As:AP-2alpha^ki, AP-2alpha^ki7lacZ, Tcfap2a^ki7lacZ

The AP-2alpha^ki knock-in/knockout allele has an IRES-lacZ cassette inserted into exon 7 of the transcription factor AP-2α gene; both abolishing endogenous gene function and placing lacZ expression under direction of the Tfap2a promoter/enhancer regions. These mice may be useful for visualizing cranial and cardiac neural crest cells in the developing embryo.

Donating Investigator

Trevor Williams, University of Colorado, Denver

Genetic overview

Genetic Background	Generation

Tfap2a^tm1Hsv

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Tfap2a	transcription factor AP-2, alpha

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Research Applications

Research Tools
Neurobiology Research
Developmental Biology Research
Cardiovascular Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The AP-2alpha^ki knock-in/knockout allele has an IRES-lacZ cassette inserted into exon 7 of the transcription factor AP-2α gene; both abolishing endogenous gene function and placing lacZ expression under direction of the Tfap2a promoter/enhancer regions. Wildtype mice have AP-2alpha expression in the neural tube, and in cranial and cardiac neural crest cells in the developing embryo, as well as in the progress zone of the limb bud, the developing kidney, the eye, and surface ectoderm. Most homozygotes exhibit prenatal or postnatal lethality due to neural tube, craniofacial, body wall, and cardiac malfomation, while the majority of heterozygotes are viable and fertile. In these mice, β-gal labels the ectoderm, and cranial and cardiac neural crest populations during the E8.5-10.5. Homozygous mice exhibit failure of neural tube closure by E9.5, and outflow tract abnormalities from E14.5-15.5.

Development

A targeting vector was designed to insert an internal ribosome entry site (IRES) and a β-galactosidase (lacZ) sequence, followed by a loxP-flanked neomycin resistance cassette, into exon 7 of the transcription factor AP-2, alpha gene (Tfap2a). The construct was electroporated into 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺-derived CJ-7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric males were bred to outbred Black Swiss females. Upon arrival, AP-2alpha^ki mice were bred to FVN/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	In mice carrying this allele ß-gal labels the ectoderm, and cranial and cardiac neural crest populations during the E8.5-10.5.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Brewer S; Jiang X; Donaldson S; Williams T; Sucov HM. 2002. Requirement for AP-2alpha in cardiac outflow tract morphogenesis. Mech Dev 110(1-2):139-49PubMed: 11744375 MGI: J:73813

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Genetics

Tfap2a^tm1Hsv

Allele Symbol: Tfap2a^tm1Hsv

Allele Name	targeted mutation 1, Henry M Sucov
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Henry M Sucov; Tfap2a^tm1Hsv
Gene Symbol and Name	Tfap2a, transcription factor AP-2, alpha
Gene Synonym(s)	AP-2 transcription factor; BOFS; AP-2; Ap-2 (a); AP-2 alpha; Ap2; AP2alpha; AP2TF; Tcfap2a; Ap2tf; Tcfap2a; AP-2alpha; TFAP2; Ap2tf
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	In mice carrying this allele ß-gal labels the ectoderm, and cranial and cardiac neural crest populations during the E8.5-10.5.
Strain of Origin	129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Chromosome	13
General Note	The authors indicate that removal of the neomycin cassette by Cre-mediated recombination does not alter gross morphology or lacZ expression pattern. (J:73813)
Molecular Note	An IRES, lacZ and floxed neomycin resistance cassette were inserted into exon 7 via homologous recombination resulting in disruption of the DNA binding domain. The mutant transcript encodes the first 6 exons of the gene followed by the IRES-lacZ message. The pattern of lacZ expression in mutant animals is similar to that of the endogenous protein.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- lacZ Expression
- Cardiovascular Research
  - endothelial cell marker (lacZ) for neovascularization
Neurobiology Research
- lacZ expression in neural tissue
- Neural Tube Defects
Developmental Biology Research
- Neural Crest Defects
- Embryonic Lethality (Homozygous)
  - incomplete
- Neural Tube Defects
Cardiovascular Research
- Heart Abnormalities

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tfap2a^tm1Hsv/Tfap2a^tm1Hsv
involves: 129S1/Sv * Black Swiss

cardiovascular system phenotype

abnormal aortic arch development

abnormal heart ventricle outflow tract morphology

double outlet right ventricle
- 8 out of 10 E14.5-15.5 embryos exhibit double outlet right ventricle (DORV)
- the remaining two E14.5-E15.5 embryos exhibit a more extreme a DORV described as tetralogy of Fallot

persistent right dorsal aorta
- double outlet right ventricle associated with a right dorsal aorta defect in 1 of 10 E14.5-15.5 embryos

pulmonary valve atresia
- complete atresia of the proximal pulmonary outflow tract is found in 1 of 10 E15.5 embryos

pulmonary valve stenosis
- extreme stenosis of the proximal pulmonary outflow tract is found in 1 of 10 E14.5 embryos

retroesophageal right subclavian artery
- retroesophageal right subclavian artery (RERSCA) is observed in 1 of 10 E14.5-15.5 embryos

ventricular septal defect
- observed in all embryos studied

craniofacial phenotype

abnormal craniofacial development
- highly disorganized craniofacial structures observed in E15.5 embryos

embryo phenotype

open neural tube
- failure of neural tube closure by E9.5

growth/size/body region phenotype

abnormal ventral body wall morphology
- failure of ventral body wall closure observed in E15.5 embryos

nervous system phenotype

open neural tube
- failure of neural tube closure by E9.5

References

Brewer S; Jiang X; Donaldson S; Williams T; Sucov HM. 2002. Requirement for AP-2alpha in cardiac outflow tract morphogenesis. Mech Dev 110(1-2):139-49PubMed: 11744375 MGI: J:73813

Additional - Tfap2a^tm1Hsv related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Generic Cre Probe
- Standard PCR:Tfap2a
- Standard PCR:Generic Cre Melt Curve Analysis
- Genotyping resources and troubleshooting
Breeding Considerations

Most homozygotes exhibit prenatal or postnatal lethality due to neural tube, craniofacial, body wall, and cardiac malfomation. The majority of heterozygotes are viable and fertile. When maintaining a live colony, heterozygotes may be bred to wildtype mice from the colony or to FVB/NJ inbred mice (Stock No. 001800).
- Additional Breeding and Husbandry Support
Citation
When using the AP-2alpha^ki mouse strain in a publication, please cite the originating article(s) and include JAX stock #023405 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wildtype for Tfap2a<tm1.1Hsv>

Related Products and Services

Frozen Mouse Embryo	STOCK Tfap2a<tm1Hsv>/J	$2595.00
Frozen Mouse Embryo	STOCK Tfap2a<tm1Hsv>/J	$2595.00
Frozen Mouse Embryo	STOCK Tfap2a<tm1Hsv>/J	$3373.50
Frozen Mouse Embryo	STOCK Tfap2a<tm1Hsv>/J	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Also Known As:AP-2alphaki, AP-2alphaki7lacZ, Tcfap2aki7lacZ

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tfap2atm1Hsv

Allele Symbol: Tfap2atm1Hsv

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tfap2atm1Hsv/Tfap2atm1Hsvinvolves: 129S1/Sv * Black Swiss

cardiovascular system phenotype

craniofacial phenotype

embryo phenotype

growth/size/body region phenotype

nervous system phenotype

References

Additional - Tfap2atm1Hsv related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As:AP-2alpha^ki, AP-2alpha^ki7lacZ, Tcfap2a^ki7lacZ

Tfap2a^tm1Hsv

Allele Symbol: Tfap2a^tm1Hsv

Genotype: Tfap2a^tm1Hsv/Tfap2a^tm1Hsv
involves: 129S1/Sv * Black Swiss

Additional - Tfap2a^tm1Hsv related