STOCK Tfap2a^tm1Hsv/J

Stock number: 023405
Product name: AP-2alpha^ki
Strain synonyms: AP-2alpha^ki7lacZ, Tcfap2a^ki7lacZ
Research areas: Cardiovascular Research, Developmental Biology Research, Neurobiology Research, Research Tools

Description

The AP-2alpha^ki knock-in/knockout allele has an IRES-lacZ cassette inserted into exon 7 of the transcription factor AP-2α gene; both abolishing endogenous gene function and placing lacZ expression under direction of the Tfap2a promoter/enhancer regions. These mice may be useful for visualizing cranial and cardiac neural crest cells in the developing embryo.

Detailed description

The AP-2alpha^ki knock-in/knockout allele has an IRES-lacZ cassette inserted into exon 7 of the transcription factor AP-2α gene; both abolishing endogenous gene function and placing lacZ expression under direction of the Tfap2a promoter/enhancer regions. Wildtype mice have AP-2alpha expression in the neural tube, and in cranial and cardiac neural crest cells in the developing embryo, as well as in the progress zone of the limb bud, the developing kidney, the eye, and surface ectoderm. Most homozygotes exhibit prenatal or postnatal lethality due to neural tube, craniofacial, body wall, and cardiac malfomation, while the majority of heterozygotes are viable and fertile. In these mice, β-gal labels the ectoderm, and cranial and cardiac neural crest populations during the E8.5-10.5. Homozygous mice exhibit failure of neural tube closure by E9.5, and outflow tract abnormalities from E14.5-15.5.

Development

A targeting vector was designed to insert an internal ribosome entry site (IRES) and a β-galactosidase (lacZ) sequence, followed by a loxP-flanked neomycin resistance cassette, into exon 7 of the transcription factor AP-2, alpha gene (Tfap2a). The construct was electroporated into 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺-derived CJ-7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric males were bred to outbred Black Swiss females. Upon arrival, AP-2alpha^ki mice were bred to FVN/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.

Allele

Symbol: Tfap2a^tm1Hsv
Name: targeted mutation 1, Henry M Sucov
MGI accession ID: MGI:2183192
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Tfap2a
Marker name: transcription factor AP-2, alpha
Chromosome: 13
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: In mice carrying this allele ß-gal labels the ectoderm, and cranial and cardiac neural crest populations during the E8.5-10.5.
Molecular note: An IRES, lacZ and floxed neomycin resistance cassette were inserted into exon 7 via homologous recombination resulting in disruption of the DNA binding domain. The mutant transcript encodes the first 6 exons of the gene followed by the IRES-lacZ message. The pattern of lacZ expression in mutant animals is similar to that of the endogenous protein.
General note: The authors indicate that removal of the neomycin cassette by Cre-mediated recombination does not alter gross morphology or lacZ expression pattern. (J:73813)