B6.Cg-Tg(CSNK1D,-EGFP)433Yfu/J

Stock number: 023404
Research areas: Neurobiology Research

Description

These transgenic mice express full-length, wildtype human CSNK1D (casein kinase 1, delta), and may be useful in studies of circadian patterns. This is line 433, carrying 2-3 copies of the transgene.

Detailed description

Mutations in the human CSNK1D (casein kinase 1, delta) gene have been associated with symptoms of Familial Advanced Sleep Phase Syndrome (FASPS). Evidence suggests that this kinase interacts with PER2 (period circadian clock 2) binding domains to regulate circadian period.

Hemizygous transgenic mice express 2-3 copies of the full-length, wildtype human gene. Under 12 hour light-12 hour dark (LD) and constant darkness (DD)conditions, these mice and T44A transgenic mutant mice (see Stock No. 022148) show similar and robust circadian rhythms of wheel-running activity. Transgenic mice carrying this wildtype form of the human gene exhibit a circadian period of approximately 23.78 hours, but when placed in combination with a Per2 S662G mutation (e.g. Stock No. 013456 or Stock No. 013700), which shortens circadian time to 22 hrs, the wildtype CSNK1D transgene further shortens the circadian period to 20.8 hours.

No phenotypic differences have been observed between this strain and founder line 432 (see Stock No. 022149).

Development

Human BAC RP11-1376P16, containing the entire CSNK1D gene on a 190-kb genomic insert (Genbank accession number AC129510) and a carboxy terminal internal ribosome entry site (IRES) followed by enhanced green fluorescent protein gene (EGFP) were introduced to C57BL/6 x SJL F1 embryos. Resultant mice were backcrossed to C57BL/6 for more than 10 generations by the donating laboratory. Fluorescence has not been directly detected in transgenic mice. This is line 433, carrying 2-3 copies of the transgene.

Allele

Symbol: Tg(CSNK1D,-EGFP)433Yfu
Name: transgene insertion 433, Ying-Hui Fu
MGI accession ID: MGI:5477832
Generation method: Transgenic
Attributes: Reporter; Inserted expressed sequence; Humanized sequence
Synonyms: Tg(CSNK1D)433Yfu
Marker symbol: Tg(CSNK1D,-EGFP)433Yfu
Marker name: transgene insertion 433, Ying-Hui Fu
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: CSNK1D,casein kinase 1 delta,human; GFP,Green Fluorescent Protein,
Site of expression: EGFP included in construct, but no EGFP signal was observed.
Molecular note: The transgene is composed of the human BAC RP11-1376P16, containing the entire CSNK1D gene on a 190-kb genomic insert (Genbank accession number AC129510) and a carboxy terminal internal ribosome entry site (IRES) followed by enhanced green fluorescent protein gene (EGFP). Lines 432 and 433 were generated.