This transgenic strain expresses human LMNB1 (lamin B1) from a FLAG-tagged tetracycline-responsive element (TRE). When crossed with animals expressing the tetracycline-controlled transactivator (tTA) or reverse tetracycline-controlled transactivator (rtTA) protein under the control of tissue-specific promoters, transgene expression can be regulated in the offspring by the administration of doxycycline.
Ying-Hui Fu, University of California, San Francisco
Louis Ptacek, University of California, San Francisco
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
Adult-onset autosomal-dominant leukodystrophy (ADLD) is a progressive and fatal neurological disorder characterized by early autonomic dysfunction, cognitive impairment, pyramidal tract and cerebellar dysfunction, and white matter loss in the central nervous system. ADLD is caused by duplication of the LMNB1 gene, which results in increased lamin B1 transcripts and protein expression.
This transgenic strain expresses human Lmnb1 from a FLAG-tagged tetracycline-responsive element (TRE). When crossed with animals expressing the tetracycline-controlled transactivator (tTA) or reverse tetracycline-controlled transactivator (rtTA) protein under the control of tissue-specific promoters, transgene expression can be regulated in the offspring by the administration of doxycycline.
When crossed with an Camk2a-tTA strain (see Stock No. 003010), LMNB1 is overexpressed specifically in neurons. When crossed with an GFAP-tTA strain (e.g. Stock No. 005964), LMNB1 is overexpressed specifically in astrocytes. Compound mutant mice show no motor deficits on the balance beam and accelerated rotarod at 12 months and no overt behavioral abnormalities beyond 1 year.
An estimated 1-2 copies of human LMNB1 is expressed from a FLAG-tagged tetracycline-responsive element (TRE) in this transgenic strain. This mutation was made and maintained on an FVB genetic background by the donating laboratory. This is founder line AF1.
Expressed Gene | LMNB1, lamin B1, human |
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Site of Expression |
Allele Name | transgene insertion AF1, Ying-Hui Fu |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | (TRE)-Lmnb1; Tg(tetO-Lmnb1)#Yfu |
Gene Symbol and Name | Tg(tetO-LMNB1)AF1Yfu, transgene insertion AF1, Ying-Hui Fu |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | LMNB1, lamin B1, human |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | The tetracyclin response element drives inducible expression of the FLAG tagged human cDNA. The founder line AF1 contains 1-2 copies of the transgene. |
Homozygotes and hemizygotes are viable and fertile.
When using the FVB-Tg(tetO-Lmnb1)AF1Yfu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023397 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(tetO-Lmnb1)AF1Yfu |
Frozen Mouse Embryo | FVB-Tg(tetO-Lmnb1)AF1Yfu/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(tetO-Lmnb1)AF1Yfu/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(tetO-Lmnb1)AF1Yfu/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(tetO-Lmnb1)AF1Yfu/J Frozen Embryo | $3373.50 |
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