B6.Cg-Stim2^tm1Rao/J

Stock number: 023351
Product name: Stim2 flox
Strain synonyms: Stim2^fl
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exon 3 of the Stim2 gene. This strain may be useful for generating conditional mutations in applications related to the regulation of cytosolic and endoplasmic reticulum calcium levels and store-operated calcium channels.

Detailed description

The targeted Stim2 gene encodes a protein involved in regulation of cytosolic and endoplasmic reticulum calcium levels and store-operated calcium channels. These Stim2^fl mice possess loxP sites on either side of exon 3 of the targeted gene. Exon 3 encodes the C-terminal calcium-binding EF hand domain. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues. Removal of the floxed sequence creates a null allele.

When bred to a strain with Cre recombinase expression in T cells (see Stock No. 017336 for example), this mutant mouse strain may be useful in studies of development of regulatory T cells.

Development

A targeting vector containing a FRT site flanked NEO cassette with a 5’ loxP site was inserted upstream of exon 3 of the Stim2 gene. A second loxP site was inserted downstream of exon 3. The construct was electroporated into B6.Cg-Thy1^a derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were bred to a FLP recombinase expressing strain, on the C57BL/6J genetic background, to excise the FRT site flanked NEO cassette. Mice that retained the loxP site flanked exon 3 were then bred to C57BL/6 mice to remove the FLPe recombinase transgene/allele. Heterozygotes were crossed to generate homozygotes. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Allele

Symbol: Stim2^tm1Rao
Name: targeted mutation 1, Anjana Rao
MGI accession ID: MGI:3778913
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Stim2
Marker name: stromal interaction molecule 2
Chromosome: 5
Strain of origin: B6.Cg-Thy1^a
Molecular note: An frt-flanked neo cassette with a 5' loxP site was inserted upstream of exon 3 and an additional loxP site was inserted downstream of exon 3. Germ-line, flp-mediated recombination was used to remove the neo cassette.