These floxed mutant mice possess loxP sites flanking exon 3 of the Stim2 gene. This strain may be useful for generating conditional mutations in applications related to the regulation of cytosolic and endoplasmic reticulum calcium levels and store-operated calcium channels.
Anjana Rao, La Jolla Institute for Allergy and Immunology
The targeted Stim2 gene encodes a protein involved in regulation of cytosolic and endoplasmic reticulum calcium levels and store-operated calcium channels.
These Stim2fl mice possess loxP sites on either side of exon 3 of the targeted gene. Exon 3 encodes the C-terminal calcium-binding EF hand domain. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.
Removal of the floxed sequence creates a null allele.
When bred to a strain with Cre recombinase expression in T cells (see Stock No. 017336 for example), this mutant mouse strain may be useful in studies of development of regulatory T cells.
A targeting vector containing a FRT site flanked NEO cassette with a 5’ loxP site was inserted upstream of exon 3 of the Stim2 gene. A second loxP site was inserted downstream of exon 3. The construct was electroporated into B6.Cg-Thy1a derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were bred to a FLP recombinase expressing strain, on the C57BL/6J genetic background, to excise the FRT site flanked NEO cassette. Mice that retained the loxP site flanked exon 3 were then bred to C57BL/6 mice to remove the FLPe recombinase transgene/allele. Heterozygotes were crossed to generate homozygotes.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Anjana Rao|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Stim2, stromal interaction molecule 2|
|Strain of Origin||B6.Cg-Thy1a|
|Molecular Note||An frt-flanked neo cassette with a 5' loxP site was inserted upstream of exon 3 and an additional loxP site was inserted downstream of exon 3. Germ-line, flp-mediated recombination was used to remove the neo cassette.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Stim2 flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #023351 in your Materials and Methods section.