JAX Mouse Strain Datasheet - 023337

B6.129S4-Cxcr3^tm1Arsa/SoghJ

Stock No:: 023337 |; CIBER

Targeted Mutation

Cryo Recovery

Overview

Also Known As: CIBER

These knock-in mice express EGFP from the endogenous X-linked Cxcr3 gene. They may be useful for tracking Cxcr3-expressing immune cells during infection and disease progression.

Donating Investigator

Abhay Satoskar, The Ohio State University Medical Center

Genetic overview

Genetic Background	Generation

Cxcr3^tm1Arsa

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter)	Cxcr3	chemokine (C-X-C motif) receptor 3

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Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

In this CIBER strain, an internal ribosome entry site (IRES), an enhanced green fluorescent protein (EGFP), and bovine growth hormone polyadenylation sequence were placed downstream of exon 2 of the X-linked chemokine (C-X-C motif) receptor 3 (Cxcr3) gene. Homozygous females and hemizygous males are viable and fertile. Cxcr3 encodes a chemokine receptor involved in immunity, autoimmunity, and progression of diseases such as cancer, and atherosclerosis. In these CIBER mice, GFP fluorescence is seen on activated Th1 cells, CD8⁺ T cells, NK cells, NKT cells, natural regulatory T cells, as well as innate CD8⁺ T cells.

Development

A targeting vector was designed to insert an internal ribosome entry site (IRES), an enhanced green fluorescent protein (EGFP), and bovine growth hormone polyadenylation sequence, followed by a frt-flanked neomycin resistance cassette, downstream of the X-linked chemokine (C-X-C motif) receptor 3 (Cxcr3) gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6-Tg(Zp3-cre)93Knw/J transgenic mice (Stock No. 003651) to delete the neo cassette. Offspring were crossed to remove the Flp-expressing transgene. These mice were bred to C57BL/6 mice for at least 10 generations (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed mixed C57BL/6J ; C57BL/6N genetic background.

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP fluorescence is seen on activated Th1 cells, CD8+ T cells, NK cells, NKT cells, natural regulatory T cells, as well as innate CD8+ T cells.

Control Suggestions

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Oghumu S; Dong R; Varikuti S; Shawler T; Kampfrath T; Terrazas CA; Lezama-Davila C; Ahmer BM; Whitacre CC; Rajagopalan S; Locksley R; Sharpe AH; Satoskar AR. 2013. Distinct Populations of Innate CD8+ T Cells Revealed in a CXCR3 Reporter Mouse. J Immunol 190(5):2229-40. PubMed: 23338236 MGI: J:193492

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Genetics

Cxcr3^tm1Arsa

Allele Symbol: Cxcr3^tm1Arsa

Allele Name	targeted mutation 1, Abhay R Satoskar
Allele Type	Targeted (Reporter)
Allele Synonym(s)	CIBER
Gene Symbol and Name	Cxcr3, chemokine (C-X-C motif) receptor 3
Gene Synonym(s)	CD182; CD183; CKR-L2; CMKAR3; Cd183; Cmkar3; Cmkar3; GPR9; Gpr9; IP10-R; Mig-R; MigR; chemokine (C-X-C) receptor 3
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	GFP fluorescence is seen on activated Th1 cells, CD8+ T cells, NK cells, NKT cells, natural regulatory T cells, as well as innate CD8+ T cells.
Strain of Origin	129S4/SvJae
Chromosome	X
Molecular Note	An IRES, EGFP and floxed pgk-neo cassette were inserted in the 3' untranslated region of exon 2. Protein expression in activated T cells from mutant mice is similar to wild-type controls. Expression pattern of EGFP matches endogenous protein expression.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
- Inflammation
Research Tools
- Genetics Research
  - Tissue/Cell Markers
  - Tissue/Cell Markers: T cell specific surface marker

Genotype: GFP related

Research Tools
- Fluorescent Proteins

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Cxcr3^tm1Arsa/Y
involves: 129S4/SvJae

normal phenotype

no abnormal phenotype detected
- viable and fertile, with no obvious abnormalities and normal T cell migration

References

Oghumu S; Dong R; Varikuti S; Shawler T; Kampfrath T; Terrazas CA; Lezama-Davila C; Ahmer BM; Whitacre CC; Rajagopalan S; Locksley R; Sharpe AH; Satoskar AR. 2013. Distinct Populations of Innate CD8+ T Cells Revealed in a CXCR3 Reporter Mouse. J Immunol 190(5):2229-40. PubMed: 23338236 MGI: J:193492

Additional - Cxcr3^tm1Arsa related

Brackett CM; Kojouharov B; Veith J; Greene KF; Burdelya LG; Gollnick SO; Abrams SI; Gudkov AV. 2016. Toll-like receptor-5 agonist, entolimod, suppresses metastasis and induces immunity by stimulating an NK-dendritic-CD8+ T-cell axis. Proc Natl Acad Sci U S A 113(7):E874-83. PubMed: 26831100 MGI: J:230433
Cohen SB; Maurer KJ; Egan CE; Oghumu S; Satoskar AR; Denkers EY. 2013. CXCR3-dependent CD4(+) T cells are required to activate inflammatory monocytes for defense against intestinal infection. PLoS Pathog 9(10):e1003706. PubMed: 24130498 MGI: J:245786
Oghumu S; Terrazas CA; Varikuti S; Kimble J; Vadia S; Yu L; Seveau S; Satoskar AR. 2015. CXCR3 expression defines a novel subset of innate CD8+ T cells that enhance immunity against bacterial infection and cancer upon stimulation with IL-15. FASEB J 29(3):1019-28. PubMed: 25466888 MGI: J:219639
Oghumu S; Varikuti S; Terrazas C; Kotov D; Nasser MW; Powell CA; Ganju RK; Satoskar AR. 2014. CXCR3 deficiency enhances tumor progression by promoting macrophage M2 polarization in a murine breast cancer model. Immunology 143(1):109-19. PubMed: 24679047 MGI: J:219472

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	X Linked - Heterozygous females and Wildtype males for Cxcr3<tm1Arsa>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Frozen Mouse Embryo

$2,595.00 per straw or vial

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Licensing Information

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Also Known As: CIBER

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Cxcr3tm1Arsa

Allele Symbol: Cxcr3tm1Arsa

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: GFP related

Mammalian Phenotype Terms by Genotype

Genotype: Cxcr3tm1Arsa/Yinvolves: 129S4/SvJae

normal phenotype

References

Additional - Cxcr3tm1Arsa related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cxcr3^tm1Arsa

Allele Symbol: Cxcr3^tm1Arsa

Genotype: Cxcr3^tm1Arsa/Y
involves: 129S4/SvJae

Additional - Cxcr3^tm1Arsa related