These knock-in mice express EGFP from the endogenous X-linked Cxcr3 gene. They may be useful for tracking Cxcr3-expressing immune cells during infection and disease progression.
Abhay Satoskar, The Ohio State University Medical Center
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Cxcr3 | chemokine (C-X-C motif) receptor 3 |
In this CIBER strain, an internal ribosome entry site (IRES), an enhanced green fluorescent protein (EGFP), and bovine growth hormone polyadenylation sequence were placed downstream of exon 2 of the X-linked chemokine (C-X-C motif) receptor 3 (Cxcr3) gene. Homozygous females and hemizygous males are viable and fertile. Cxcr3 encodes a chemokine receptor involved in immunity, autoimmunity, and progression of diseases such as cancer, and atherosclerosis. In these CIBER mice, GFP fluorescence is seen on activated Th1 cells, CD8+ T cells, NK cells, NKT cells, natural regulatory T cells, as well as innate CD8+ T cells.
A targeting vector was designed to insert an internal ribosome entry site (IRES), an enhanced green fluorescent protein (EGFP), and bovine growth hormone polyadenylation sequence, followed by a frt-flanked neomycin resistance cassette, downstream of the X-linked chemokine (C-X-C motif) receptor 3 (Cxcr3) gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to
C57BL/6-Tg(Zp3-cre)93Knw/J transgenic mice (Stock No. 003651) to delete the neo cassette. Offspring were crossed to remove the Flp-expressing transgene. These mice were bred to C57BL/6 mice for at least 10 generations (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP fluorescence is seen on activated Th1 cells, CD8+ T cells, NK cells, NKT cells, natural regulatory T cells, as well as innate CD8+ T cells. |
Allele Name | targeted mutation 1, Abhay R Satoskar |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | CIBER |
Gene Symbol and Name | Cxcr3, chemokine (C-X-C motif) receptor 3 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP fluorescence is seen on activated Th1 cells, CD8+ T cells, NK cells, NKT cells, natural regulatory T cells, as well as innate CD8+ T cells. |
Strain of Origin | 129S4/SvJae |
Chromosome | X |
Molecular Note | An IRES, EGFP and floxed pgk-neo cassette were inserted in the 3' untranslated region of exon 2. Protein expression in activated T cells from mutant mice is similar to wild-type controls. Expression pattern of EGFP matches endogenous protein expression. |
This gene is X-linked. When maintaining a live colony, homozygous females may be bred with hemizygous males.
When using the CIBER mouse strain in a publication, please cite the originating article(s) and include JAX stock #023337 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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X Linked - Heterozygous females and Wildtype males for Cxcr3<tm1Arsa>, |
Frozen Mouse Embryo | B6.129S4-Cxcr3<tm1Arsa>/SoghJ | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Cxcr3<tm1Arsa>/SoghJ | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Cxcr3<tm1Arsa>/SoghJ | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Cxcr3<tm1Arsa>/SoghJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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