These Homer3 KO mice may be useful for studying the role of HOMER3 on neurochemical defects.
Paul Worley, Johns Hopkins University School of Medic
These Homer3 KO mice have a neomycin cassette inserted into exon 3 of homer homolog 3 (Homer3) gene, abolishing gene expression. HOMER3 is a postsynaptic density scaffolding protein expressed in the central nervous system, as well as skeletal muscle, where it mediates the formation of large macromolecular complexes and regulates group 1 metabotrophic glutamate receptor function. They do not display any abnormalities in cognitive, motivational or emotional processing.
A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 3 of the homer homolog 3 (Homer3) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6J females. The donating investigator reports that these mutant mice were then backcrossed to C57BL/6J mice for at least 5 generations (see SNP results below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 27 markers throughout the genome were segregating for an unknown background, and one marker was segregating for 129. This suggested an incomplete backcross. Aldo all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Marlin H Dehoff|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Homer3, homer scaffolding protein 3|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A PGK-neo was inserted into exon 3.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Homer3 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #023314 in your Materials and Methods section.