Overview

These Homer2 KO mice may be useful for studying the role of HOMER2 on neurochemical defects.

Donating Investigator

Paul Worley, Johns Hopkins University School of Medic

Genetic overview

Genetic Background	Generation

Homer2^tm1Mhd

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Homer2	homer scaffolding protein 2

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Research Applications

Neurobiology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

These Homer2 KO mice have a neomycin cassette inserted into exon 3 of homer homolog 2 (Homer2) gene, abolishing gene expression. HOMER2 is a postsynaptic density scaffolding protein expressed in the central nervous system, as well as skeletal muscle, where it mediates the formation of large macromolecular complexes and regulates group 1 metabotrophic glutamate receptor function. Mice that are homozygous for this allele are viable and fertile. These mice avoid drinking high concentrations of ethanol (EtOH) and do not develop place preference or locomotor sensitization after administration. They display an increase in sensitivity to cocaine-induced locomotion and conditioned reward. They do not display any abnormalities in cognitive, motivational or emotional processing.

Development

A targeting vector was designed to insert a neomycin resistance (neo) cassette, in reverse orientation, into exon 3 of the homer homolog 2 (Homer2) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6J females. The donating investigator reports that these mutant mice were then backcrossed to C57BL/6J mice for at least 5 generations (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Suggestions

Approximate Controls

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Shin DM; Dehoff M; Luo X; Kang SH; Tu J; Nayak SK; Ross EM; Worley PF; Muallem S. 2003. Homer 2 tunes G protein-coupled receptors stimulus intensity by regulating RGS proteins and PLCbeta GAP activities. J Cell Biol 162(2):293-303PubMed: 12860966 MGI: J:95213

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Genetics

Homer2^tm1Mhd

Allele Symbol: Homer2^tm1Mhd

Allele Name	targeted mutation 1, Marlin H Dehoff
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	H2ko
Gene Symbol and Name	Homer2, homer scaffolding protein 2
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	7
Molecular Note	A PGK-neomycin gene was inserted in inverse orientation into exon 3. mRNA and protein products could not be detected.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Behavioral and Learning Defects
  - genes regulating preferences to alcohol
- Neurotransmitter Receptor and Synaptic Vesicle Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Homer2^tm1Mhd/Homer2^tm1Mhd
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

endocrine/exocrine gland phenotype

abnormal pancreas physiology
- carbachol stimulated acinar cells showed a higher increase in intracellular calcium concentration due to increased calcium release from internal stores than controls
- two-fold increased frequency of intracellular calcium oscillations in acinar cells in response to stimulation with bombesin or CCK compared to controls

The following phenotype relates to a compound genotype created using this strain

Genotype: Homer2^tm1Mhd/Homer2^tm1Mhd
B6.129-Homer2

behavior/neurological phenotype

enhanced behavioral response to cocaine
- capacity of acute cocaine to elicit locomotor hyperactivity is enhanced in mutants compared to controls

abnormal associative learning
- mice exhibit cocaine-induced place conditioning (increased time spent in environment containing stimulus/reward) at doses that do not elicit place conditioning in controls
- mutants acquire operant self-administration of cocaine more readily than controls
- mutants exhibit cocaine-induced place conditioning compared to wild-type controls

abnormal cocaine consumption
- mutants acquire operant self-administration of cocaine more readily than controls

enhanced conditioned place preference behavior
- mice prefer to spend more time in chamber where cocaine is available compared to controls

mammalian phenotype

behavior/neurological phenotype

nervous system phenotype

abnormal nervous system physiology
- mutants show lower (~50%) extracellular glutamate levels in the nucleus accumbens compared to wild-type mice; injection of cocaine into the nucleus accumbens causes elevation of extracellular glutamate levels in mutants but not wild-type controls

References

Shin DM; Dehoff M; Luo X; Kang SH; Tu J; Nayak SK; Ross EM; Worley PF; Muallem S. 2003. Homer 2 tunes G protein-coupled receptors stimulus intensity by regulating RGS proteins and PLCbeta GAP activities. J Cell Biol 162(2):293-303PubMed: 12860966 MGI: J:95213

Additional - Homer2^tm1Mhd related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Homer2-Alternate 1
- Standard PCR:Generic Neo
- Probe:Generic Neo
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the B6.129(Cg)-Homer2^tm1Mhd/PfwJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #023313 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wild-type for Homer2<tm1Mhd>

Related Products and Services

Frozen Mouse Embryo	B6.129(Cg)-Homer2<tm1Mhd>/PfwJ	$2595.00
Frozen Mouse Embryo	B6.129(Cg)-Homer2<tm1Mhd>/PfwJ	$2595.00
Frozen Mouse Embryo	B6.129(Cg)-Homer2<tm1Mhd>/PfwJ	$3373.50
Frozen Mouse Embryo	B6.129(Cg)-Homer2<tm1Mhd>/PfwJ	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Homer2tm1Mhd

Allele Symbol: Homer2tm1Mhd

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Homer2tm1Mhd/Homer2tm1Mhdinvolves: 129S1/Sv * 129X1/SvJ * C57BL/6

endocrine/exocrine gland phenotype

Genotype: Homer2tm1Mhd/Homer2tm1MhdB6.129-Homer2

behavior/neurological phenotype

mammalian phenotype

nervous system phenotype

References

Additional - Homer2tm1Mhd related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Homer2^tm1Mhd

Allele Symbol: Homer2^tm1Mhd

Genotype: Homer2^tm1Mhd/Homer2^tm1Mhd
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Genotype: Homer2^tm1Mhd/Homer2^tm1Mhd
B6.129-Homer2

Additional - Homer2^tm1Mhd related