B6.129S-Sfrp1^tm1Jrbn/PcmaJ

Stock number: 023209
Research areas: Developmental Biology Research, Internal/Organ Research, Research Tools

Description

A lacZ-neo cassette replaces exon 1 of the secreted frizzled-related protein 1 (Sfrp1) gene. These mice may be useful for studying developing prostate.

Detailed description

In this strain, a lacZ-neo cassette replaces exon 1 of the secreted frizzled-related protein 1 (Sfrp1) gene, abolishing gene function. SFRP1 is a stromal-to-epithelial paracrine signaling molecule expressed in the developing mesenchyme/stroma of the prostate from p1 to p21. It is also a modulator of Wnt signaling, disruption of which has been associated with cancer progression. Homozygotes are viable and fertile. Sfrp1^lacZ mice exhibit multiple prostatic developmental defects including reduced branching morphogenesis, and delayed proliferation. Increased expression of genes encoding prostate-specific secretory proteins such as beta-microseminoprotein (Msmb), spermine binding protein (Sbp), and tranglutaminase 4 (Tgm4) is also seen in these mice. β-gal activity is evident in the developing mesenchyme/stroma of the prostate gland from p1 to p21. β-gal activity also was seen in various locations during embryonic development, with low levels detected as early as the 3-somite stage in the extra-embryonic yolk sac and in the neural plate

Development

A targeting vector was designed to replace exon 1 of the secreted frizzled-related protein 1 (Sfrp1) gene with a β-galactosidase (lacZ)-neomycin resistance cassette. The construct was electroporated into 129S/Sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6J females. These mice were bred to C57BL/6NCrl mice for at least 5 generations using a marker-assisted, speed congenic approach. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.

Allele

Symbol: Sfrp1^tm1Jrbn
Name: targeted mutation 1, Jeffrey Rubin
MGI accession ID: MGI:3796251
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Sfrp1
Marker name: secreted frizzled-related protein 1
Chromosome: 8
Strain of origin: 129/Sv
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: β-gal activity is evident in the developing mesenchyme/stroma of the prostate gland from p1 to p21. β-gal activity also was seen in various locations during embryonic development, with low levels detected as early as the 3-somite stage in the extra-embryonic yolk sac and in the neural plate.
Molecular note: A lacZ-neo cassette was inserted downstream of the initiation methionine codon in exon 1 replacing the endogenous sequence. The absence of protein product was confirmed by western blot analysis on prostate and seminal vesicle extracts.