A lacZ-neo cassette replaces exon 1 of the secreted frizzled-related protein 1 (Sfrp1) gene. These mice may be useful for studying developing prostate.
Jeffrey S. Rubin, National Cancer Institute
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Sfrp1 | secreted frizzled-related protein 1 |
In this strain, a lacZ-neo cassette replaces exon 1 of the secreted frizzled-related protein 1 (Sfrp1) gene, abolishing gene function. SFRP1 is a stromal-to-epithelial paracrine signaling molecule expressed in the developing mesenchyme/stroma of the prostate from p1 to p21. It is also a modulator of Wnt signaling, disruption of which has been associated with cancer progression. Homozygotes are viable and fertile. Sfrp1lacZ mice exhibit multiple prostatic developmental defects including reduced branching morphogenesis, and delayed proliferation. Increased expression of genes encoding prostate-specific secretory proteins such as beta-microseminoprotein (Msmb), spermine binding protein (Sbp), and tranglutaminase 4 (Tgm4) is also seen in these mice. β-gal activity is evident in the developing mesenchyme/stroma of the prostate gland from p1 to p21. β-gal activity also was seen in various locations during embryonic development, with low levels detected as early as the 3-somite stage in the extra-embryonic yolk sac and in the neural plate
A targeting vector was designed to replace exon 1 of the secreted frizzled-related protein 1 (Sfrp1) gene with a β-galactosidase (lacZ)-neomycin resistance cassette. The construct was electroporated into 129S/Sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6J females. These mice were bred to C57BL/6NCrl mice for at least 5 generations using a marker-assisted, speed congenic approach. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | β-gal activity is evident in the developing mesenchyme/stroma of the prostate gland from p1 to p21. β-gal activity also was seen in various locations during embryonic development, with low levels detected as early as the 3-somite stage in the extra-embryonic yolk sac and in the neural plate. |
Allele Name | targeted mutation 1, Jeffrey Rubin |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Sfrp1lacZ; Sfrp1tm1Pcma |
Gene Symbol and Name | Sfrp1, secreted frizzled-related protein 1 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | β-gal activity is evident in the developing mesenchyme/stroma of the prostate gland from p1 to p21. β-gal activity also was seen in various locations during embryonic development, with low levels detected as early as the 3-somite stage in the extra-embryonic yolk sac and in the neural plate. |
Strain of Origin | 129/Sv |
Chromosome | 8 |
Molecular Note | A lacZ-neo cassette was inserted downstream of the initiation methionine codon in exon 1 replacing the endogenous sequence. The absence of protein product was confirmed by western blot analysis on prostate and seminal vesicle extracts. |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129S-Sfrp1tm1Jrbn/PcmaJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #023209 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Sfrp1<tm1Jrbn> |
Frozen Mouse Embryo | B6.129S-Sfrp1<tm1Jrbn>/PcmaJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Sfrp1<tm1Jrbn>/PcmaJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S-Sfrp1<tm1Jrbn>/PcmaJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S-Sfrp1<tm1Jrbn>/PcmaJ Frozen Embryo | $3373.50 |
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