These floxed mutant mice possess loxP sites flanking exon 1 of the X-linked Zic3 gene and may be useful in applications related to the study of embryonic development.
John Belmont, Baylor College of Medicine
These Zic3flox mice possess loxP sites flanking exon 1 of the X-linked zinc finger protein of the cerebellum 3 (Zic3) gene. ZIC3 is a transcription factor involved in early stages of gastrulation, and central nervous system, cardiac and left-right axial development. Zic3flox/flox females and Zic3flox/y males are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting double mutant offspring will have exon 1 deleted in the cre-expressing tissue(s).
For example, when bred to B6.Cg-Tg(Sox2-cre)1Amc/J transgenic mice (Stock No. 008454) expressing cre in epiblast cells, resulting offspring exhibit 'kinked' tails, anus and eye anomalies, cardiac looping defects, cleft palate, exencephaly, abnormal lung position, and axial skeleton defects leading to hind-limb paralysis.
A targeting vector was designed to insert a frt-flanked PGK-neo cassette followed by a loxP site upstream of exon 1, and a second loxP site downstream of exon 1 of the X-linked zinc finger protein of the cerebellum 3 (Zic3) gene. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric males were bred to B6(Cg)-Tyrc-2J/J females (Stock No. 000058). Heterozygous females were crossed to FLPe males on a 129/Sv background to remove the neo cassette, and offspring were crossed to remove the Flp-expressing transgene. Zic3flox/flox females and Zic3flox/y males were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation and the Tyrc-2J allele was selected against.
|Allele Name||targeted mutation 2.1, John W Belmont|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Zic3, zinc finger protein of the cerebellum 3|
|Strain of Origin||129S7/SvEvBrd-Hprtb-m2|
|Molecular Note||An FRT-flanked neo cassette with a 3' loxP site was inserted upstream of exon 1. An additional loxP site was inserted downstream of exon 1. Flp-mediated recombination removed the neo cassette and left exon 1 floxed.|
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129-Zic3tm2.1Jwb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023162 in your Materials and Methods section.