B6;129S-Gad2^tm1.1Ksvo/J

Stock number: 023140
Product name: Gad2-T2a-NLS-mCherry
Research areas: Neurobiology Research, Research Tools

Description

These Gad2-T2a-NLS-mCherry mice have mCherry expression directed to glutamic acid decarboxylase 2 (Gad2)-expressing cells by the endogenous promoter/enhancer elements.

Detailed description

Mice that are homozygous for the Gad2-T2a-NLS-mCherry mutant allele are viable and fertile. This allele harbors a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus) and a nuclear localized mCherry in the 3' UTR of the glutamic acid decarboxylase 2 (Gad2) gene. GAD2 catalyzes the production of gamma-aminobutyric acid from L-glutamic acid, and is expressed in the brain and pancreas. In these mice, mCherry expression is directed by the endogenous Gad2 promoter/enhancer elements.

Development

A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), and a nuclear localized mCherry in the 3' UTR of the glutamic acid decarboxylase 2 (Gad2) gene. A frt-flanked neo was placed downstream of the mCherry. This construct was electroporated into 129S6-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred with C57BL/6J mice. Offspring were bred to B6.129S4-Gt(ROSA)26Sor^tm1(FLP1)Dym/RainJ mice (Stock No. 009086) to remove the neo, and resulting mice were bred to C57BL/6J mice. These mice were crossed to remove the Flp-expressing transgene. Upon arrival at The Jackson Laboratory, these mice were bred to C57BL/6J mice (Stock No. 000664) for at least one generation to establish a colony.

Allele

Symbol: Gad2^tm1.1Ksvo
Name: targeted mutation 1.1, Karel Svoboda
MGI accession ID: MGI:5512722
Generation method: Targeted
Attributes: Reporter
Marker symbol: Gad2
Marker name: glutamic acid decarboxylase 2
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Expressed genes: RFP,Red Fluorescent Protein,coral
Molecular note: A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), and a nuclear localized mCherry in in-frame after the last codon of the Gad2 gene. A frt-flanked neo was places downstream of the mCherry. Flp-mediated recombination removed the neomycin resistance cassette.