These Gad2-T2a-NLS-mCherry mice have mCherry expression directed to glutamic acid decarboxylase 2 (Gad2)-expressing cells by the endogenous promoter/enhancer elements.
Karel Svoboda, Janelia Farm Research Campus
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Gad2 | glutamic acid decarboxylase 2 |
Mice that are homozygous for the Gad2-T2a-NLS-mCherry mutant allele are viable and fertile. This allele harbors a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus) and a nuclear localized mCherry in the 3' UTR of the glutamic acid decarboxylase 2 (Gad2) gene. GAD2 catalyzes the production of gamma-aminobutyric acid from L-glutamic acid, and is expressed in the brain and pancreas. In these mice, mCherry expression is directed by the endogenous Gad2 promoter/enhancer elements.
A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), and a nuclear localized mCherry in the 3' UTR of the glutamic acid decarboxylase 2 (Gad2) gene. A frt-flanked neo was placed downstream of the mCherry. This construct was electroporated into 129S6-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred with C57BL/6J mice. Offspring were bred to B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ mice (Stock No. 009086) to remove the neo, and resulting mice were bred to C57BL/6J mice. These mice were crossed to remove the Flp-expressing transgene. Upon arrival at The Jackson Laboratory, these mice were bred to C57BL/6J mice (Stock No. 000664) for at least one generation to establish a colony.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression |
Allele Name | targeted mutation 1.1, Karel Svoboda |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | Gad22A-mCherry; Gad2-2A-nls-mcherry; Gad2-T2A-NLS-mCherry |
Gene Symbol and Name | Gad2, glutamic acid decarboxylase 2 |
Gene Synonym(s) | |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), and a nuclear localized mCherry in in-frame after the last codon of the Gad2 gene. A frt-flanked neo was places downstream of the mCherry. Flp-mediated recombination removed the neomycin resistance cassette. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Gad2-T2a-NLS-mCherry mouse strain in a publication, please cite the originating article(s) and include JAX stock #023140 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Gad2<tm1.1Ksvo> |
Frozen Mouse Embryo | B6;129S-Gad2<tm1.1Ksvo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S-Gad2<tm1.1Ksvo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S-Gad2<tm1.1Ksvo>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S-Gad2<tm1.1Ksvo>/J Frozen Embryo | $3373.50 |
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