A1-a KO mice have a neoR cassette replacing exon 1 of the Bcl2a1a gene, encoding the Bcl2- (B cell leukemia/lymphoma 2)-related protein A1-a. In homozygotes, expression of A1-a is abolished, while expression of the alternative A1 isoforms (A1-b, A1-d) is intact. A1-a is an anti-apoptotic member of the Bcl-2 family that is up-regulated in inflammatory myeloid cells. Homozygotes are viable and fertile. When infected intraperitoneally with Toxoplasma gondii these mice exhibit an attenuation of inflammation, they show no differences in peritoneal parasite load. These mice also survive one day longer than WT littermates after infection.
A targeting vector was designed to replace exon 1 of the targeted gene, as well as 386 bp of 5' flanking sequence, with a neoR cassette in reverse orientation to the gene. The construct was electroporated into WW6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females. These A1-a ko mice were bred to C57BL/6J for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Michael Prystowsky|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||A1-a-; Bcl2a1atm1Orlf|
|Gene Symbol and Name||Bcl2a1a, B cell leukemia/lymphoma 2 related protein A1a|
|Strain of Origin||STOCK 129/Sv and C57BL/6J and SJL|
|Molecular Note||The gene was disrupted by replacement of exon 1 with a neomycin resistance cassette via homologous recombination. Absence of gene expression was confirmed by RT-PCR analysis of bone marrow macrophage RNA from homozygous mutant animals.|
When maintaining a live colony, homozygous mice may be bred together.
When using the A1-a- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37468 in your Materials and Methods section.